Silicon application enhances resistance against sorghum mosaic virus infection in sugarcane

Mosaic disease is one of the predominant viral diseases affecting sugarcane plants worldwide. In China, it is mainly caused by the sorghum mosaic virus (SrMV). Exogenous silicon (ESi) has emerged as a promising inducer of plant disease resistance. This study aims to elucidate the mechanistic effects of exogenous silicon on enhancing the resistance to SrMV in sugarcane. ESi was applied to the soil at different rates (15 g per barrel^-1, 30 g per barrel^-1, and 45 g per barrel^-1), leading to a significant reduction in disease incidence (26.66-82.22%) compared to non-silicon-treated plants. Silicon application (15 g ESi barrel^-1, 30 g ESi barrel^-1) mitigated SrMV inhibition of sugarcane growth, including plant height, stem diameter, and leaf area, while improving photosynthesis, including stomatal conductance, intercellular CO₂ concentration, net photosynthetic rate, and transpiration rate. Additionally, silicon mitigates SrMV-induced degradation of chlorophyll a and b and carotenoid content, alongside heightened activities of superoxide dismutase, peroxidase, and catalase, and decreased content of malondialdehyde and hydrogen peroxide in sugarcane leaves. The ultrastructural analysis revealed silicon's capacity to reduce SrMV accumulation within sugarcane mesophyll cells while preserving chloroplast integrity. Additionally, silicon application increases SA content in sugarcane leaves and upregulates the expression of key SA pathway genes (PAL, PR1, NPR1). These findings suggest that silicon may contribute to sugarcane resistance to SrMV by potentially influencing antioxidant enzyme activity, ROS production, and SA pathway genes.