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Histopathological parameters and immunoexpression of CD45RO (CLA) and CD68 in chronic rhinosinusitis with nasal
Irina Enache1, Alex Emilian Stepan, Florin Anghelina
1Department of Otorhinolaryngology, University of Medicine and Pharmacy of Craiova, Romania; carmen_mogo@yahoo.com.
Abstract:
Nasal polyps develop as a result of inflammation of the nasal and sinus mucosa. Allergies and nasal infections cause inflammation, and these are the main reasons why these symptoms appear in the first place. This study highlights the involvement of macrophages, as well as T- and B-lymphocytes, in the pathophysiology of nasal polyps. For the evaluation of lymphocyte activity, we analyzed the immunoexpression of cluster of differentiation 45RO [CD45RO; common leukocyte antigen (CLA)] and for macrophages we analyzed the immunoexpression of cluster of differentiation 68 (CD68). Our research, conducted on 110 sinonasal polyps harvested from chronic rhinosinusitis patients with nasal polyps, focused on analyzing both the epithelial and stromal compartments in relation to pre-established composite scores. Additionally, specific histopathological parameters were included in the study. We concluded that the inflammatory cells were more prevalent in the stromal compartment compared to the epithelial compartment. The statistical evaluation of CD45RO (CLA) and CD68 scores in the stromal compartment were also associated with high histological composite scores.
Insights
Nasal polyps involve inflammation driven by immune cells like macrophages and lymphocytes. These inflammatory cells, identified by CD68 and CD45RO markers, are more concentrated in the polyp
Area of Science:
- Immunology
- Pathophysiology
- Rhinology
Background:
- Nasal polyps result from chronic inflammation of the nasal and sinus mucosa, often triggered by allergies or infections.
- Immune cell involvement, particularly macrophages and lymphocytes, is crucial in the development of nasal polyps.
Purpose of the Study:
- To investigate the role of macrophages and lymphocytes in the pathophysiology of nasal polyps.
- To analyze the immunoexpression of CD68 (macrophages) and CD45RO (lymphocytes) in sinonasal polyps.
- To correlate immune cell distribution and expression levels with histopathological parameters.
Main Methods:
- Analysis of 110 sinonasal polyp samples from patients with chronic rhinosinusitis.
- Immunohistochemical evaluation of CD68 and CD45RO (common leukocyte antigen) expression.
- Assessment of immune cell distribution in epithelial and stromal compartments.
- Correlation with histopathological composite scores and specific parameters.
Main Results:
- Inflammatory cells, including macrophages and lymphocytes, were significantly more prevalent in the stromal compartment than the epithelial compartment.
- Higher scores for CD45RO (CLA) and CD68 immunoexpression in the stroma were associated with elevated histological composite scores.
- Specific histopathological parameters were also considered in the analysis.
Conclusions:
- Immune cell infiltration, particularly macrophages and T-lymphocytes (CD45RO+), is a key feature in the stroma of nasal polyps.
- The density of these inflammatory cells correlates with the severity of the disease as indicated by histopathological scoring.
- Understanding immune cell roles provides insights into nasal polyp pathogenesis and potential therapeutic targets.
