Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Precision bioprinting-based extrusion of tumour spheroids on pre-matured<i>in vitro</i>tissue models on demand.

Biofabrication·2026
Same author

Investigating reported self-efficacy, attitudes, challenges, and knowledge of physical educators of autistic children across Europe.

International journal of developmental disabilities·2026
Same author

Droplet Microfluidics-Assisted Fabrication of Magnetite Nanoparticle Hybrid Microgels for Facile Protein Immobilization.

Chembiochem : a European journal of chemical biology·2026
Same author

Scale-Specific Viscoelastic Characterization of Hydrogels: Integrated AFM and Finite Element Modeling.

Small (Weinheim an der Bergstrasse, Germany)·2025
Same author

Expanding the Usage of Lignin in DLP 3D Printing by Optimized Synthesis and Processing Parameters.

ACS applied polymer materials·2025
Same author

Step Test for Rapid Screening of Material and Process Parameters for Resin Development in DLP 3D Printing.

Angewandte Chemie (International ed. in English)·2025

Related Experiment Video

Updated: May 27, 2025

Synthesis of an Intein-mediated Artificial Protein Hydrogel
15:06

Synthesis of an Intein-mediated Artificial Protein Hydrogel

Published on: January 27, 2014

12.1K

Regulating Protein Immobilization During Cell-Free Protein Synthesis in Hyaluronan Microgels.

Anika Kaufmann1, Kateryna Ivanova1, Julian Thiele1,2

  • 1Leibniz-Institut für Polymerforschung Dresden e. V., Hohe Straße 6, 01069, Dresden, Germany.

Advanced Biology
|February 17, 2025
PubMed
Summary

Researchers developed cell-like microgel platforms for synthetic biology. These platforms enable controlled, in situ protein synthesis and accumulation, paving the way for temporal-spatial regulation in artificial environments.

Keywords:
GFPbifunctional microgelscell‐free protein synthesisprotein immobilization

More Related Videos

Author Spotlight: A Novel Approach for Embedding Cell-Free Protein Synthesis Reactions in Hydrogels
06:38

Author Spotlight: A Novel Approach for Embedding Cell-Free Protein Synthesis Reactions in Hydrogels

Published on: June 23, 2023

1.1K
OaAEP1-Mediated Enzymatic Synthesis and Immobilization of Polymerized Protein for Single-Molecule Force Spectroscopy
08:34

OaAEP1-Mediated Enzymatic Synthesis and Immobilization of Polymerized Protein for Single-Molecule Force Spectroscopy

Published on: February 5, 2020

6.7K

Related Experiment Videos

Last Updated: May 27, 2025

Synthesis of an Intein-mediated Artificial Protein Hydrogel
15:06

Synthesis of an Intein-mediated Artificial Protein Hydrogel

Published on: January 27, 2014

12.1K
Author Spotlight: A Novel Approach for Embedding Cell-Free Protein Synthesis Reactions in Hydrogels
06:38

Author Spotlight: A Novel Approach for Embedding Cell-Free Protein Synthesis Reactions in Hydrogels

Published on: June 23, 2023

1.1K
OaAEP1-Mediated Enzymatic Synthesis and Immobilization of Polymerized Protein for Single-Molecule Force Spectroscopy
08:34

OaAEP1-Mediated Enzymatic Synthesis and Immobilization of Polymerized Protein for Single-Molecule Force Spectroscopy

Published on: February 5, 2020

6.7K

Area of Science:

  • Synthetic biology
  • Biomaterials science
  • Chemical engineering

Background:

  • Cell-like platforms are crucial for mimicking biological processes in artificial systems.
  • Investigating the interplay between protein synthesis and accumulation within confined environments is essential for synthetic biology applications.

Purpose of the Study:

  • To develop and characterize bifunctional microgels as a platform for studying cell-free protein synthesis (CFPS) and in situ protein accumulation.
  • To investigate the temporal-spatial regulation of protein synthesis and binding within a microgel matrix.

Main Methods:

  • Fabrication of hyaluronic acid (HA) microgels functionalized with nitrilotriacetic acid (NTA) moieties.
  • Optimization of cell-free protein synthesis (CFPS) using linear DNA templates.
  • Functionalization of microgels with DNA templates and Ni2+-activated NTA for binding His-tag modified proteins.
  • Real-time monitoring of CFPS and protein accumulation within the microgels.

Main Results:

  • Characterization of HA-microgel capacity for binding His-tag modified Green Fluorescent Protein (GFP).
  • Optimization of CFPS conditions for efficient protein production within the microgel system.
  • Demonstration of in situ synthesis and accumulation of GFP-His within the functionalized microgels.
  • Observation of GFP-His binding to the microgel platform over time.

Conclusions:

  • The developed microgel system serves as a novel platform for studying protein synthesis and accumulation in a controlled, artificial environment.
  • This approach enables the investigation of temporal-spatial regulation of protein synthesis through tailored binding or release mechanisms.
  • The study lays the groundwork for advanced synthetic biology applications utilizing microgel-based reaction environments.