Comparative evaluation of apical microleakage of mineral trioxide aggregate, Biodentine, and Bio-C Repair as root-end...

Root-end filling materials enhance the apical sealing ability of the root canal to avoid leakage of irritants and to prevent the reentering of microorganisms.

The objective of the present study was to compare and evaluate apical microleakage in retrograde filling materials with mineral trioxide aggregate (MTA), Biodentine, and Bio-C Repair using an ultraviolet (UV)-visible spectrophotometer.

Fifty maxillary incisors were selected and decoronated. Instrumentation was done with ProTaper rotary files and obturated with AH Plus sealer and ProTaper gutta-percha cone using lateral condensation technique. The apical part of each root was resected at 90° to the long axis of the root for 3 mm, and retrograde cavity preparation was done up to 3 mm using an ultrasonic retro tip. After conditioning the root end with 17% ethylenediaminetetraacetic acid, the teeth were divided into four groups. Group 1 = Positive Control (n = 5), Group 2 was filled with MTA (n = 15), Group 3 was filled with Biodentine (n = 15), and Group 4 was filled with Bio-C Repair (n = 15). All samples were incubated in 5 mL of 2% methylene blue dye for 72 h after which teeth were immersed in 65% nitric acid for 72 h. The solutions were then filtered using fine grit filter paper and centrifuged at 3500 rpm for 5 min. The solution thus collected was used to determine absorbency in UV-visible spectrophotometer at 550 nm.

The data were analyzed using one-way analysis of variance and Tukey post hoc tests. P <0.05 was considered significant for all analyses.

The results showed that Biodentine had the least dye absorbance, which means less microleakage, and there was no significant difference between MTA and Bio-C Repair.

Biodentine showed superior sealing ability as a retrograde filling material compared to Bio-C Repair and MTA.