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Reverse Genetics Mediated Recovery of Infectious Murine Norovirus
Published on: June 24, 2012
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TREM-1 as a Potential Coreceptor in Norovirus Pathogenesis: Insights from Transcriptomic Analysis and Molecular
Mike Telemaco Contreras Colmenares1, Amanda de Oliveira Matos1, Pedro Henrique Dos Santos Dantas1
1Laboratório de Imunologia de Mucosas e Imunoinformática, Instituto de Patologia Tropical e Saúde Pública, Universidade Federal de Goiás, Goiânia 74605-170, Brazil.
ACS Omega
|February 17, 2025
Summary
Toll-like receptor expressed in myeloid cells 1 (TREM-1) may play a role in norovirus (NoV) infection. This study found TREM-1 interacts with the NoV VP1 protein, suggesting its involvement in disease.
Area of Science:
- Virology
- Immunology
- Bioinformatics
Background:
- Norovirus (NoV) causes acute diarrheal disease, but its host interactions are challenging to study due to cultivation difficulties.
- Toll-like receptor expressed in myeloid cells 1 (TREM-1) is implicated in amplifying inflammatory responses during viral infections.
Purpose of the Study:
- To investigate the potential involvement of TREM-1 in norovirus infection and pathogenesis.
- To elucidate the molecular interactions between TREM-1 and norovirus proteins.
Main Methods:
- Analysis of public transcriptomic data to assess TREM-1 expression during murine norovirus (MNoV) infection.
- Molecular docking and dynamics simulations to model interactions between murine and human TREM-1 and MNoV/NoV VP1 proteins.
- Coexpression analysis of TREM-1 with apoptosis and pyroptosis pathway genes during MNoV infection.
Main Results:
- Increased expression of Trem1 and Trem3 observed during MNoV infection.
- Murine TREM-1 recognized the C'-D' segment of MNoV VP1.
- Human TREM-1 showed a conserved interaction with NoV GII.4 VP1 protein segments (S and P) across different years, mediated by the CDR3 region.
- Trem1 coexpressed primarily with pyroptosis pathway genes during MNoV infection.
Conclusions:
- TREM-1 interacts with the norovirus VP1 protein, suggesting its involvement in NoV pathogenesis.
- TREM-1 may function as a coreceptor in norovirus infections.
- The interaction between TREM-1 and NoV is conserved across different viral strains and time periods.
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