Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Comparative Diagnostic Performance of Vibration Perception Thresholds and clinical indicators in Sensorineural Hand-Arm Vibration Syndrome. A Study of Sensitivity, Specificity, Predictive value, Accuracy and ROC AUC.

Journal of occupational and environmental medicine·2026
Same author

Surgically treated metacarpal fractures in adults: a study with 3286 cases based on the Swedish National Quality Registry for Hand Surgery.

BMC musculoskeletal disorders·2026
Same author

Surgery for unmeasurable neuropathy to alleviate non-specific upper extremity pain: round table discussion.

The Journal of hand surgery, European volume·2026
Same author

Family history of diabetes in first-degree relatives and risk of hand surgical diagnoses: a Swedish population-based cohort study from the Malmö Diet and Cancer Study.

BMJ open·2026
Same author

A fovea-centered retinal signature linked to plasma biomarkers in prodromal Alzheimer's disease.

Alzheimer's & dementia (Amsterdam, Netherlands)·2026
Same author

A laboratory micro-CT technique is useful to visualize and characterize dermal skin components in a 3D manner.

Experimental and therapeutic medicine·2026

Related Experiment Video

Updated: May 27, 2025

Axoplasm Isolation from Rat Sciatic Nerve
05:29

Axoplasm Isolation from Rat Sciatic Nerve

Published on: September 24, 2010

13.6K

Refinement of Protein Extraction Protocols for Human Peripheral Nerve Tissue.

Drifa Frostadottir1,2, Charlotte Welinder3, Raquel Perez1,4

  • 1Department of Translational Medicine - Hand Surgery, Lund University, Malmö S-20502, Sweden.

ACS Omega
|February 17, 2025
PubMed
Summary

The optimal method for extracting proteins from fresh-frozen human peripheral nerves involves using Ripa buffer with 8 M urea and the Bullet Blender, requiring at least 1.2 mg of sample for consistent results.

More Related Videos

Optimization of Laser-Capture Microdissection for the Isolation of Enteric Ganglia from Fresh-Frozen Human Tissue
08:28

Optimization of Laser-Capture Microdissection for the Isolation of Enteric Ganglia from Fresh-Frozen Human Tissue

Published on: June 14, 2018

8.6K
Expression, Isolation, and Purification of Soluble and Insoluble Biotinylated Proteins for Nerve Tissue Regeneration
12:03

Expression, Isolation, and Purification of Soluble and Insoluble Biotinylated Proteins for Nerve Tissue Regeneration

Published on: January 22, 2014

33.5K

Related Experiment Videos

Last Updated: May 27, 2025

Axoplasm Isolation from Rat Sciatic Nerve
05:29

Axoplasm Isolation from Rat Sciatic Nerve

Published on: September 24, 2010

13.6K
Optimization of Laser-Capture Microdissection for the Isolation of Enteric Ganglia from Fresh-Frozen Human Tissue
08:28

Optimization of Laser-Capture Microdissection for the Isolation of Enteric Ganglia from Fresh-Frozen Human Tissue

Published on: June 14, 2018

8.6K
Expression, Isolation, and Purification of Soluble and Insoluble Biotinylated Proteins for Nerve Tissue Regeneration
12:03

Expression, Isolation, and Purification of Soluble and Insoluble Biotinylated Proteins for Nerve Tissue Regeneration

Published on: January 22, 2014

33.5K

Area of Science:

  • Biochemistry
  • Proteomics
  • Neuroscience

Background:

  • Accurate protein extraction from human peripheral nerve tissue is crucial for understanding neurological diseases.
  • Existing protein extraction methods may not be optimized for the unique composition of peripheral nerves.
  • Standardization of protein extraction protocols is needed for reproducible proteomic analyses.

Purpose of the Study:

  • To develop an effective protein extraction method for freshly frozen human peripheral nerves.
  • To determine the minimum sample amount required for consistent protein extraction.
  • To compare different extraction methods for maximizing protein identification.

Main Methods:

  • Five protein extraction methods were evaluated using Ripa buffer and 8 M urea.
  • Extraction was performed using either a Bullet Blender or a Bioruptor.
  • Sample input varied from 0.6 mg to 10 mg to determine optimal quantity.

Main Results:

  • Ripa buffer with Bioruptor or Bullet Blender identified 60% and 62% of total proteins, respectively.
  • 8 M urea with Bullet Blender yielded 55% protein identification, while Bioruptor yielded 39%.
  • Combining Ripa buffer, 8 M urea, and Bullet Blender identified 81% of total proteins (2126 identities).
  • Consistent protein extraction outcomes were achieved with sample amounts ≥1.2 mg.

Conclusions:

  • The combination of Ripa buffer, 8 M urea, and Bullet Blender is the most effective method for protein extraction from human peripheral nerves.
  • A minimum sample weight of 1.2 mg is recommended for reproducible protein extraction.
  • This optimized method enhances proteomic analysis by increasing the number of identified proteins, including those in the extracellular matrix (ECM).