Related Experiment Video
Updated: Apr 26, 2026

A Tandem Liquid Chromatography–Mass Spectrometry-based Approach for Metabolite Analysis of Staphylococcus aureus
Published on: March 28, 2017
Untargeted metabolomic analysis uncovers metabolic variability of four Bifidobacterial strains for probiotic
Kailong Liu1,2,3,4, Guoqiang Yao1,2,3,4, Zhan Yang1,2,3,4
1Key Laboratory of Dairy Biotechnology and Engineering, Ministry of Education, Inner Mongolia Agricultural University, Hohhot, China.
Abstract:
Bifidobacterium species are essential members of the human gut microbiota, playing crucial roles in host health. Variations in the metabolic functions of different Bifidobacterium strains can have distinct health effects, making it essential to understand their metabolic characteristics for the development of targeted probiotic formulations. In this study, we cultivated four selected Bifidobacterium strains from three species: Bifidobacterium animalis subsp. lactis BB-69, Bbm-19, Bifidobacterium brevis BX-18, and Bifidobacterium longum subsp. infantis B8762. We conducted an untargeted comparative metabolomic analysis to investigate the intracellular metabolic profile and functional activities of these strains. A total of 1,340 metabolites were identified, revealing significant variations in their metabolomic profiles. Notably, Bifidobacterium animalis subsp. lactis Bbm-19 showed higher activity in amino acid biosynthesis, while Bifidobacterium animalis subsp. lactis BB-69 exhibited increased activity in secondary bile acid biosynthesis and alpha-linolenic acid metabolism. Bifidobacterium longum subsp. infantis B8762 demonstrated enhanced activity in polycyclic aromatic hydrocarbon degradation, vitamin digestion and absorption, and galactose metabolism. Bifidobacterium breve BX-18 was more active in tryptophan metabolism and pentose and glucuronate interconversions. Targeted analyses of specific metabolites further revealed strain-specific metabolic pathways. This study systematically elucidates the metabolic profile differences among the four investigated Bifidobacterium strains at the untargeted metabolomic level, providing valuable insights into their metabolic characteristics and potential applications in probiotic development.
More Related Videos
Related Concept Videos
Self-Evaluation: Self-Enhancement and Self-Verification
General State of Stress
Specifically, consider a tetrahedral element where one face, labeled XYZ, is perpendicular to the line OA, and the remaining faces align with the coordinate axes with point O as the origin. At any point, such as point O, the stress tensor can be used to determine the stress...
Ethnic Identity within a Larger Culture
Introduction to Stress and Lifestyle
Psychological Responses to Stress
Stress and Mental Health
Individuals with depression often experience challenges in both their personal and professional...

