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Published on: September 14, 2013
Isolation and identification of a native bacterium Citrobacter farmeri against microcystin-LR in anaerobic
Rong Zhang1, Lili Yang1, Sihong Long1
1Hunan Province Key Laboratory of Typical Environmental Pollution and Health Hazards, School of Public Health, Hengyang Medical School, University of South China, Hengyang, China.
Abstract:
Microcystin-LR (MC-LR), produced by cyanobacterial harmful algal blooms, poses a serious threat to aquatic ecosystems and human health. Biodegradation is an important method for MC-LR elimination. Aerobic biodegradation has been extensively studied and many bacteria were identified. However, few MC-degrading bacteria have been isolated from anaerobic environments, and these degradation mechanisms are poorly understood. The aim of this study was to collect anaerobic MC-degrading bacteria from lake sediments in Lake Taihu using acclimation culture to assess biodegradation. Five strains with MC-LR degradation ability were isolated, with strain A4 belonging to Citrobacter farmeri (C. farmeri). exhibiting the highest efficiency at 0.486 μg/ml/d. High-performance liquid chromatography (HPLC) identified two novel MC-LR degradation products. Further polymerase chain reaction (PCR) analysis suggested that C. farmeri A4 did not possess the known MC-degrading gene mlrABCD, suggesting the involvement of an mlrABCD-independent anaerobic degradation pathway. Data demonstrated that the bacterial strain C. farmeri A4 found in Lake Taihu exhibited high anaerobic MC-LR degradation properties, which indicated that anaerobic biodegradation may constitute an important biological method for MC-LR removal in natural environment.
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