Single-Cell/Particle Sample Introduction Device for Mass Cytometry Based on Gas-Driven Flow Focusing.

Area of Science:

• Biotechnology
• Analytical Chemistry
• Single-cell analysis

Background:

• Mass cytometry offers advanced multi-marker analysis but suffers from sample introduction issues like clogging.
• Conventional nebulizers in mass cytometry systems are prone to clogging, hindering long-term sample analysis.

Purpose of the Study:

• To develop a novel, clog-free sample introduction device for mass cytometry.
• To improve the reliability and ease of use of mass cytometry systems for single-cell and single-particle analysis.

Main Methods:

• Developed a plug-and-play device utilizing a gas-driven flow focusing protocol with a virtual hole.
• Achieved single-cell monodispersion via liquid jet breakup, bypassing conventional nebulization.
• Employed high-speed microscopy to study liquid jet dynamics and used inductively coupled plasma mass spectrometry for validation.

Main Results:

• The developed device successfully generated single-cell monodispersion without clogging.
• Comparative analysis showed consistent results between the new device and conventional nebulizers.
• The device demonstrated high universality and compatibility with mass cytometry and ICP-MS instruments.

Conclusions:

• The novel flow focusing device provides a reliable, clog-free solution for mass cytometry sample introduction.
• This innovation enhances the practicality of long-term, high-throughput single-cell analysis.
• The device maintains analytical performance while significantly reducing operational challenges.