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Generation of CNPY3 knock out cell line in the H1 (WA01) hESC background
Xing Qi1, Feng Yao2, Shan Yongli2
1Guangdong Provincial Key Laboratory of Stem Cell and Regenerative Medicine, Guangzhou Institutes of Biomedicine and Health, Chinese Academy of Sciences, Guangzhou 510530, China; Centre for Regenerative Medicine and Health, Hong Kong Institute of Science and Innovation, Chinese Academy of Sciences, Hong Kong.
Abstract:
The CNPY3 gene encodes a protein that interacts with members of the toll-like receptor (TLR) protein family and functions as a chaperone, aiding in the proper folding and export of these proteins. We generated a homozygous CNPY3 knockout human embryonic stem cell (hESC) line WAe001-A-2T (H1-CNPY3-/-), using CRISPR/Cas9 genome editing technology. The WAe001-A-2T cell line exhibited a normal karyotype and maintained the typical characteristics of undifferentiated hESCs, including pluripotent gene expression and differentiation potential in vivo. The CNPY3 knockout cell line serves as a valuable resource for investigating the role of the CNPY3 gene in embryonic development and lineage differentiation in vitro.
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