Blunted cortisol as a biomarker of depression based on the attenuation hypothesis: A Mendelian randomization analysis...

Both elevated and blunted cortisol responses have been associated with depression. Previous Mendelian randomization (MR) studies have largely ruled out cortisol as a cause of depression. Based on the attenuation hypothesis, this MR study used depression as exposure to assess whether cortisol might be a consequence and therefore a biomarker of depression.

Strong (P < 5 × 10^-8) and independent (r² < 0.001) single nucleotide polymorphisms (SNPs) associated with broadly defined depression (294,322 cases, 741,438 controls) were used as instruments. These were applied to genetic associations with morning, fasting, and random plasma cortisol in the CORtisol NETwork (CORNET) consortium (n = 25,314), METabolic Syndrome in Men (METSIM) study (n = 6667), and Canadian Longitudinal Study on Aging (CLSA) cohort (n = 8299). Multivariable MR, adjusting for childhood maltreatment and major mental disorders, was conducted to address potential horizontal pleiotropy from dichotomous depression. Instruments were also selected by evidence of colocalization with major depressive disorder to address non-specificity.

Using 133 SNPs as instruments, depression was inversely associated with morning plasma cortisol (β per log-odds of genetic liability to depression = -0.107 [95 % CI, -0.181 to -0.032]) in the CORNET consortium. Replication in the METSIM study (β = -0.203 [95 % CI, -0.367 to -0.040]) and CLSA cohort (β = -0.091 [95 % CI, -0.220 to 0.039]) showed consistent but not always significant associations. Multivariable MR and follow-up analysis incorporating colocalization supported these findings.

Consistent with the attenuation hypothesis, blunted cortisol response appeared to be a consequence and potentially a biomarker of depression. Future studies are needed to provide more interpretable effect sizes and validate other biomarker measures.