Optimization of bioactive compounds extraction from Red Cardinal grape pomace using choline chloride and lactic...

Area of Science:

• Agricultural Chemistry
• Biotechnology
• Food Science

Background:

• Grape pomace is a rich source of bioactive compounds, often underutilized.
• Efficient extraction methods are needed to harness its potential for various industries.

Purpose of the Study:

• To optimize the extraction of bioactive compounds from grape pomace.
• To investigate the use of deep eutectic solvents (DES) for enhanced extraction.
• To evaluate the antioxidant and antibacterial properties of the extracted compounds.

Main Methods:

• Response surface methodology (RSM) with a central composite design was employed.
• Key extraction parameters optimized included solvent-to-material ratio, temperature, and time.
• Deep eutectic solvents composed of choline chloride and lactic acid were utilized.

Main Results:

• Optimal extraction conditions: 25:1 (v·w^-1) ratio, 70°C, 118 min.
• High yields obtained: 81.90 mg GAE·g^-1 DW total phenolics, 174.66 mg AE·g^-1 DW total saponins, 31.13 mg CE·g^-1 DW total proanthocyanidins.
• Extract showed significant antioxidant capacity and antibacterial activity against tested microbial strains.

Conclusions:

• The optimized DES extraction method is effective for recovering valuable bioactive compounds from grape pomace.
• The grape pomace extract possesses potent antioxidant and antibacterial properties.
• The extract holds promise for applications in the food, nutraceutical, and cosmetic industries.