Related Experiment Video
Updated: May 27, 2025

Divergence of Root Microbiota in Different Habitats based on Weighted Correlation Networks
Published on: September 25, 2021
OsNLP3 and OsPHR2 orchestrate direct and mycorrhizal pathways for nitrate uptake by regulating NAR2.1-NRT2s complexes
Shuangshuang Wang1, Hanghang Ye1, Congfan Yang1
1State Key Laboratory of Crop Genetics & Germplasm Enhancement and Utilization, Zhongshan Biological Breeding Laboratory, Department of Plant Nutrition, College of Resources and Environmental Sciences, Nanjing Agricultural University, Nanjing 210095, China.
Abstract:
Nitrogen (N) is the most important essential nutrient required by plants. Most land plants have evolved two N uptake pathways, a direct root pathway and a symbiotic pathway, via association with arbuscular mycorrhizal (AM) fungi. However, the interaction between the two pathways is ambiguous. Here, we report that OsNAR2.1-OsNRT2s, the nitrate (NO3-) transporter complexes with crucial roles in direct NO3- uptake, are also recruited for symbiotic NO3- uptake. OsNAR2.1 and OsNRT2.1/2.2 are coregulated by NIN-like protein 3 (OsNLP3), a key regulator in NO3- signaling, and OsPHR2, a major regulator of phosphate starvation responses. More importantly, AM symbiosis induces expression of OsNAR2.1-OsNRT2s, OsNLP3, and OsSPX4, encoding an intracellular Pi sensor, in arbuscular-containing cells, but weakens their expression in the epidermis. OsNAR2.1 and OsNLP3 can activate both mycorrhizal NO3- uptake and mycorrhization efficiency. Overall, we demonstrate that OsNLP3 and OsPHR2 orchestrate the direct and mycorrhizal NO3- uptake pathways by regulating the NAR2.1-NRT2s complexes in rice.
More Related Videos
10:58Plant Promoter Analysis: Identification and Characterization of Root Nodule Specific Promoter in the Common Bean
Published on: December 23, 2017
11:33Investigating Interactions Between Histone Modifying Enzymes and Transcription Factors in vivo by Fluorescence Resonance Energy Transfer
Published on: October 14, 2022
Related Concept Videos
Key Elements for Plant Nutrition
The Roles of Bacteria and Fungi in Plant Nutrition
Responses to Salt Stress
Cell Signaling in Plants
Short-distance Transport of Resources
Tonicity in Plants
Tonicity
Tonicity describes the capacity of a cell to lose or gain water depending on the solute...