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Related Experiment Video

Updated: May 27, 2025

Using Expansion Microscopy to Physically Enlarge Whole-Mount Drosophila Embryos for Super-Resolution Imaging
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Nanoscale Resolution Imaging of Whole Mouse Embryos Using Expansion Microscopy.

Jueun Sim1, Chan E Park1, In Cho1

  • 1Department of Materials Science and Engineering, Korea Advanced Institute of Science and Technology, Daejeon 34141, Republic of Korea.

ACS Nano
|February 18, 2025
PubMed

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Summary
This summary is machine-generated.

Whole-body expansion microscopy (ExM) enables nanoscale imaging of entire embryonic and neonatal mice by overcoming challenges with calcified tissues. This cyclic digestion technique allows for 4-fold expansion, revealing detailed anatomical and protein structures.

Area of Science:

  • Biotechnology
  • Microscopy
  • Developmental Biology

Background:

  • Nanoscale imaging of whole vertebrates is crucial for understanding human diseases.
  • Expansion microscopy (ExM) offers potential but faces challenges with calcified tissues in later developmental stages.

Purpose of the Study:

  • To develop a novel ExM technique for super-resolution imaging of whole embryonic and neonatal mice.
  • To overcome limitations in expanding tissues with high calcification.

Main Methods:

  • Introduced whole-body ExM utilizing cyclic digestion with alternating enzyme compositions.
  • Repeated cycles of digestion allowed for expansion of mouse embryos up to E18.5 and neonatal mice.
  • Validated the retention of fluorescent protein signals for neuronal structure visualization.
Keywords:
embryo imagingexpansion microscopyperipheral nerve systemsuper-resolution imagingwhole-body imaging

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Main Results:

  • Achieved 4-fold expansion of embryonic and neonatal mice, enabling volumetric imaging.
  • Successfully expanded tissues with significant calcification without extensive optimization.
  • Demonstrated visualization of neuronal structures in transgenic mice using retained fluorescent protein signals.

Conclusions:

  • Whole-body ExM is a powerful technique for nanoscale imaging of whole vertebrates.
  • This method facilitates the study of molecular and anatomical changes in developing and neonatal mammals.
  • Potential to advance research in developmental biology and disease mechanisms across vertebrates.