Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Reusable 3D-printed microfluidic-on-fabric with modular electrodes for point-of-care Na<sup>+</sup> and K<sup>+</sup> detection in various biofluids.

Analytical and bioanalytical chemistry·2026
Same author

Glutamine enhances endothelial cell survival and vasodilation by increasing glutathione to reduce oxidative stress.

Physiological reports·2026
Same author

A customizable continuous and near real-time TEER platform to study anti-cancer drug toxicity in barrier tissues.

Journal of pharmaceutical analysis·2026
Same author

Extracellular Matrix Microstructures Directly Regulate Glutathione Bioavailability in Human Hepatocytes.

Biomacromolecules·2025
Same author

3D Printed Transwell Microfluidic Devices for Epithelial Cell Culture with Shear Stress.

ACS measurement science au·2025
Same author

Microfluidic Determination of Cell-Derived ATP and Single Cell Pressure Mapping Confirms Benefits of Normoglycemic Stored Red Blood Cells.

ACS measurement science au·2025

Related Experiment Video

Updated: May 27, 2025

A Versatile Automated Platform for Micro-scale Cell Stimulation Experiments
12:21

A Versatile Automated Platform for Micro-scale Cell Stimulation Experiments

Published on: August 6, 2013

10.5K

3D-Printed Microfluidic-Based Cell Culture System With Analysis to Investigate Macrophage Activation.

Major A Selemani1, Giraso Keza Monia Kabandana2, Chengpeng Chen2

  • 1Department of Chemistry, Saint Louis University, St. Louis, Missouri, USA.

Electrophoresis
|February 18, 2025
PubMed
Summary

This study developed 3D-printed microfluidic devices for cell culture, enabling rapid analysis of cellular processes. Larger silk fibers in the devices increased nitric oxide and itaconate production in macrophages.

More Related Videos

Production and Characterization of Human Macrophages from Pluripotent Stem Cells
08:05

Production and Characterization of Human Macrophages from Pluripotent Stem Cells

Published on: April 16, 2020

16.3K
Reprograming Model of Human Monocyte-derived Macrophages for In-vitro Assays
08:37

Reprograming Model of Human Monocyte-derived Macrophages for In-vitro Assays

Published on: April 18, 2025

188

Related Experiment Videos

Last Updated: May 27, 2025

A Versatile Automated Platform for Micro-scale Cell Stimulation Experiments
12:21

A Versatile Automated Platform for Micro-scale Cell Stimulation Experiments

Published on: August 6, 2013

10.5K
Production and Characterization of Human Macrophages from Pluripotent Stem Cells
08:05

Production and Characterization of Human Macrophages from Pluripotent Stem Cells

Published on: April 16, 2020

16.3K
Reprograming Model of Human Monocyte-derived Macrophages for In-vitro Assays
08:37

Reprograming Model of Human Monocyte-derived Macrophages for In-vitro Assays

Published on: April 18, 2025

188

Area of Science:

  • Biomedical Engineering
  • Cell Biology
  • Analytical Chemistry

Background:

  • Studying intracellular and extracellular processes requires dynamic analysis.
  • Microfluidic devices offer precise control over cellular environments.
  • Macrophage polarization is crucial in tissue engineering and immune responses.

Purpose of the Study:

  • To develop and validate 3D-printed microfluidic cell culture devices for dynamic analysis.
  • To investigate the impact of extracellular matrix fiber size on macrophage polarization.
  • To enable rapid, quantitative measurement of key analytes.

Main Methods:

  • Fabrication of 3D-printed microfluidic devices with integrated circulation.
  • On-line quantification of nitric oxide production using amperometric flow cells.
  • Off-line quantification of intracellular itaconate production using LC/MS.
  • Culturing macrophages on silk fibers of varying sizes (512 nm vs. 1280 nm).

Main Results:

  • Demonstrated real-time and rapid off-line analyte quantification within minutes.
  • Observed increased nitric oxide and itaconate production with larger silk fibers (1280 nm) compared to smaller fibers (512 nm).
  • Successfully correlated extracellular matrix properties with macrophage response.

Conclusions:

  • 3D-printed microfluidic devices coupled with circulation systems are effective for studying dynamic cellular processes.
  • Extracellular matrix fiber size significantly influences macrophage polarization and key analyte production.
  • This platform provides a foundation for advanced microfluidic 3D cell culture with integrated analysis for flow-dependent processes.