Related Experiment Video
Updated: May 27, 2025
04:41
Isolation, Culture, and Characterization of Primary Dermal Fibroblasts from Human Keloid Tissue
Published on: July 28, 2023
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Specific Non-Coding RNAs Involve in and Regulate the Transcriptional Network during Keloid Formation
Xun Zhang1, Linlin Song2, Yong Ma1
1Department of Burns and Plastic Surgery, General Hospital of Ningxia Medical University, Yinchuan, Ningxia, China.
Critical Reviews in Eukaryotic Gene Expression
|February 18, 2025
Summary
This study reveals that long non-coding RNAs (lncRNAs) and microRNAs (miRNAs) are key regulators in keloid formation. These non-coding RNAs (ncRNAs) form a complex network influencing immune cell infiltration and disease development.
Area of Science:
- Dermatology and Molecular Biology
- Genomics and Bioinformatics
Background:
- Keloid formation is a detrimental wound healing disorder with largely unknown molecular regulators.
- Non-coding RNAs (ncRNAs), including long non-coding RNAs (lncRNAs) and microRNAs (miRNAs), are implicated in various biological processes.
Purpose of the Study:
- To identify dysregulated lncRNAs and miRNAs in keloid formation.
- To elucidate the regulatory network and molecular functions of these ncRNAs in keloid pathogenesis.
- To investigate the relationship between ncRNAs and immune cell infiltration in keloids.
Main Methods:
- Integrated analysis of RNA-sequencing and miRNA microarray datasets from keloid-prone and healthy individuals.
- Bioinformatic construction of lncRNA-immune cell infiltration networks and competing endogenous RNA (ceRNA) networks.
- Identification of key lncRNAs and miRNAs involved in keloid pathogenesis.
Main Results:
- Identified 583 keloid-specific lncRNAs and 104 miRNAs, many associated with ossification.
- Found specific dysregulation of macrophages, NK cells, and dendritic cells in keloid development.
- Constructed a regulatory network involving 20 lncRNAs and identified two hub lncRNAs (DLEU1, SLC25A21-AS1) and five hub miRNAs (miR-197-5p, miR-940, miR-6765-5p, miR-711, miR-4284).
Conclusions:
- lncRNAs and miRNAs play significant roles in keloid pathogenesis.
- A complex regulatory network of lncRNAs and miRNAs is involved in keloid formation and immune infiltration.
- Identified potential therapeutic targets, including hub lncRNAs and miRNAs, for keloid management.
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