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Overview Of Cell Separation And Isolation

Overview Of Cell Separation And Isolation

Cell separation was first achieved in 1964 by S. H. Seal, who separated large tumor cells from the smaller blood cells using filtration. Two years later, Pohl and Hawk performed experiments on how cells respond differently to a nonuniform electric field based on the cell type. Such observations were the inception of cell separation methods, which allow isolating a single cell type from a heterogeneous sample.

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Related Experiment Video

Updated: May 4, 2026

Separating Beads and Cells in Multi-channel Microfluidic Devices Using Dielectrophoresis and Laminar Flow

Separating Beads and Cells in Multi-channel Microfluidic Devices Using Dielectrophoresis and Laminar Flow

Published on: February 4, 2011

Characterizing Recent PDMS Changes in Electrokinetic-Based Microfluidic Devices' Performance and Manufacturing for

Alexandra R Hyler¹, Dean E Thomas¹, Kyle S Kinskie¹

¹CytoRecovery, Inc., Blacksburg, Virginia, USA.

Electrophoresis

|February 18, 2025

Summary

This summary is machine-generated.

Researchers identified unexpected PDMS material changes causing bubble formation and cell death in microfluidic devices. Robust quality control and bubble removal protocols enabled consistent cell sorting performance, crucial for commercialization.

Keywords:

cell sorting commercialization electrokinetics microfabrication microfluidics

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Last Updated: May 4, 2026

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Polydimethylsiloxane-polycarbonate Microfluidic Devices for Cell Migration Studies Under Perpendicular Chemical and Oxygen Gradients

Polydimethylsiloxane-polycarbonate Microfluidic Devices for Cell Migration Studies Under Perpendicular Chemical and Oxygen Gradients

Published on: February 23, 2017

Area of Science:

Biotechnology
Materials Science
Cell Biology

Background:

Microfluidic devices offer precise cell sorting without labeling.
Commercialization of microfluidic devices is limited by fabrication challenges.
Contactless dielectrophoresis (cDEP) is a promising label-free cell enrichment technique.

Purpose of the Study:

Investigate a label-free cDEP microfluidic device for cell subpopulation enrichment.
Identify the root cause of dynamic bubble formation impacting device performance.
Assess the feasibility of scaling cDEP device manufacturing.

Main Methods:

Systematic evaluation of manufacturing, environmental, and experimental variables.
Chemical analysis of polydimethylsiloxane (PDMS) material.
Comparison of alternate polymers for device fabrication.
Development of bubble removal protocols for cDEP devices.

Main Results:

Unexpected bubble formation led to nearly 100% cell death within minutes.
Investigated variables, including alternate polymers, did not resolve the issue.
Chemical analysis revealed altered PDMS properties and composition as the cause.
Developed protocols achieved consistent performance with >90% cell viability.

Conclusions:

Altered PDMS properties are a critical factor affecting cDEP device performance.
Robust quality control and bubble management are essential for reliable microfluidic cell sorting.
Addressing PDMS material consistency is key for commercializing cDEP technology.