Reprogramming Lung Redox Homeostasis by NIR Driven Ultra-Small Pd Loaded Covalent Organic Framework Inhibits NF-κB...

Area of Science:

• Biomedical Engineering
• Nanotechnology
• Immunotherapy

Background:

• Acute lung injury (ALI) is characterized by inflammation and oxidative stress.
• Reprogramming lung redox homeostasis is a promising therapeutic strategy for ALI.
• Reactive oxygen species (ROS) play a critical role in ALI pathogenesis.

Purpose of the Study:

• To develop a novel nanozyme-based therapy for ALI.
• To investigate the efficacy of TP@Pd combined with near-infrared (NIR) irradiation for ALI treatment.
• To elucidate the underlying mechanisms of TP@Pd+NIR in ameliorating lung injury.

Main Methods:

• Synthesis of ultra-small Palladium (Pd) loaded covalent organic framework (TP@Pd).
• Evaluation of TP@Pd nanozyme activity under NIR irradiation.
• Assessment of therapeutic effects in an ALI rat model, including biochemical assays, histological analysis, and immune cell profiling.

Main Results:

• TP@Pd nanoparticles exhibited nanozyme functionalities and broad-spectrum ROS scavenging.
• The TP@Pd+NIR strategy significantly reduced ROS and inflammatory cytokines (IL-6, TNF-α, IL-1β).
• Treatment promoted M2 macrophage polarization, enhanced immunoregulation, and accelerated tissue repair by upregulating SOD2, HSP70, and CD31.

Conclusions:

• The TP@Pd+NIR strategy offers a synergistic approach for ALI amelioration.
• This novel therapy effectively alleviates lung inflammation and promotes tissue repair.
• This approach holds potential for treating ROS-related diseases beyond ALI.