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Published on: May 17, 2016
miR-196-5p regulates myogenesis and induces slow-switch fibers formation by targeting PBX1
Yufei Wang1, Donghao Zhang1, Songhang Yu1
1State Key Laboratory of Swine and Poultry Breeding Industry, Key Laboratory of Livestock and Poultry Multiomics, Ministry of Agriculture and Rural Affairs, Farm Animal Genetic Resources Exploration and In-novation Key Laboratory of Sichuan Province, College of Animal Science and Technology, Sichuan Agricultural University, Chengdu 611130, China.
Abstract:
Skeletal muscle, which is crucial for meat production, color, and quality is regulated by complex genetic mechanisms. MicroRNAs (miRNA), serve a crucial part in regulating skeletal muscle myogenesis together with the switching of muscle fiber types, but the identification of key miRNAs and their underlying molecular mechanism has been hindered. In the present study, miRNA sequencing was utilized to identify the differentially expressed miRNAs (DEMs) in different skeletal muscles, among which miR-196-5p was found notably upregulated in chicken soleus (SOL) muscles, suggesting the potential role of miR-196-5p in slow-switch fiber formation. Next, the gain- and loss-of-function experiments confirmed the inhibitory role and stimulatory effects of miR-196-5p on myoblast expansion, myotube maturation, and slow-switch myofibers formation, respectively. Through integrated bioinformatics and experimental analysis, the interaction between miR-196-5p and PBX1 was additionally clarified. PBX1 exhibits a promotive role in skeletal muscle myogenesis, while it exerts an inhibitory effect during the formation of slow-switch myofibers. In conclusion, we propose that miR-196-5p has an important involvement in modulating of skeletal muscle structural composition and function.
Insights
This study reveals microRNAs (miRNAs) regulate skeletal muscle development. Specifically, miR-196-5p promotes slow-twitch muscle fiber formation by interacting with PBX1, impacting muscle composition and function.
Area of Science:
- Molecular Biology
- Genetics
- Animal Science
Background:
- Skeletal muscle characteristics crucial for meat production are genetically controlled.
- MicroRNAs (miRNAs) are key regulators of muscle development and fiber type switching.
- Identifying specific miRNAs and their mechanisms in skeletal muscle remains challenging.
Purpose of the Study:
- To identify differentially expressed miRNAs (DEMs) in chicken skeletal muscles.
- To elucidate the role of miR-196-5p in skeletal muscle myogenesis and fiber type determination.
- To investigate the molecular mechanism of miR-196-5p, including its interaction with PBX1.
Main Methods:
- miRNA sequencing to identify DEMs.
- Gain- and loss-of-function experiments to assess miR-196-5p activity.
- Bioinformatics and experimental analyses to clarify miRNA-target interactions.
Main Results:
- miR-196-5p was significantly upregulated in chicken soleus (SOL) muscles.
- miR-196-5p inhibits myoblast expansion and myotube maturation but promotes slow-twitch myofiber formation.
- PBX1 promotes myogenesis but inhibits slow-twitch myofiber formation, interacting with miR-196-5p.
Conclusions:
- miR-196-5p plays a critical role in regulating skeletal muscle structural composition and function.
- The miR-196-5p/PBX1 interaction is a key mechanism influencing slow-twitch muscle fiber development.
- This research provides insights into the genetic control of muscle quality traits.
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