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Referenced-Closed Bipolar Electrode to Enable the Photoelectrochemical-Electrochromic Synchronous Biosensing
Shuyun Meng1, Na Dong1, Shuda Liu1
1Laboratory of Modern Agricultural Equipment and Technology, Ministry of Education, School of Agricultural Engineering, Jiangsu University, Zhenjiang, Jiangsu 212013, China.
Analytical Chemistry
|February 18, 2025
Summary
A novel referenced-closed bipolar electrode (ref-CBPE) platform enhances photoelectrochemical (PEC)-electrochromic biosensors by stabilizing the electrolyte environment. This dual-modal biosensor achieves a 40-fold photocurrent amplification for sensitive detection.
Area of Science:
- Electrochemistry
- Biosensing
- Materials Science
Background:
- Electrolyte solution conditions (salinity, pH, ascorbic acid) can destabilize electrochromic materials in photoelectrochemical (PEC)-electrochromic biosensors.
- Conventional biosensors face challenges in maintaining material stability and achieving sensitive, dual-modal detection.
Purpose of the Study:
- To develop a referenced-closed bipolar electrode (ref-CBPE)-enabled PEC-electrochromic biosensing platform for synchronous dual-modal detection.
- To enhance the stability of electrochromic materials against electrolyte interference and improve biosensor performance.
- To demonstrate a portable and accurate biosensing device for target protein detection.
Main Methods:
- Development of a ref-CBPE platform to drive redox reactions, isolating the electrochromic material from electrolyte interference.
- Utilized type II CdSe quantum dots (QDs)/ZnO heterojunctions as the anode and polyaniline as the cathode for synchronous PEC and electrochromic signal acquisition.
- Constructed a miniaturized, portable dual-modal biosensing device using 3D printing technology for Cry1Ab protein detection.
Main Results:
- The ref-CBPE configuration significantly improved signal response and achieved a 40-fold photocurrent amplification compared to conventional CBPEs.
- The biosensor demonstrated high portability, selectivity, and accuracy in detecting the model target protein (Cry1Ab).
- Synchronous acquisition of both PEC and electrochromic signals was successfully achieved.
Conclusions:
- The ref-CBPE-enabled PEC-electrochromic biosensing platform offers a robust solution for stable and sensitive dual-modal detection.
- This approach overcomes electrolyte interference issues, paving the way for advanced portable biosensing devices.
- The developed technology provides a new avenue for creating portable PEC-electrochromic biosensors with dual-signal synchronous acquisition capabilities.
