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Regulation of co-translational mRNA decay by PAP and DXO1 in Arabidopsis

Marie-Christine Carpentier1,2, Anne-Elodie Receveur1,2, Adrien Cadoudal1,2

  • 1CNRS-LGDP UMR 5096, 58 avenue Paul Alduy, Perpignan, 66860, France.

BMC Plant Biology
|February 18, 2025
PubMed
Abstract

Insights

The co-translational mRNA decay pathway (CTRD) is regulated by exoribonucleases like XRN4 and DXO1 in plants. FRY1 inactivation impairs CTRD, while PAP treatment stabilizes CTRD targets.

Area of Science:

  • Plant molecular biology
  • Gene expression regulation
  • RNA metabolism

Background:

  • mRNA decay is crucial for cellular homeostasis.
  • The co-translational mRNA decay pathway (CTRD) is an evolutionarily conserved mechanism in eukaryotes, particularly important in plants.
  • XRN4 is a key exoribonuclease involved in CTRD in Arabidopsis, but its regulation is not fully understood.

Purpose of the Study:

  • To investigate the molecular regulation of the CTRD pathway in Arabidopsis.
  • To explore the role of 3'-phosphoadenosine 5'-phosphate (PAP) and associated exoribonucleases in CTRD regulation.

Main Methods:

  • Utilized the 5'Pseq approach to assess CTRD activity.
  • Investigated the effects of FRY1 inactivation and exogenous PAP treatment on CTRD mRNA targets.
  • Examined the involvement of exoribonuclease DXO1 in the CTRD pathway.

Main Results:

  • FRY1 inactivation was found to impair XRN4-mediated CTRD activity.
  • Exogenous PAP treatment demonstrated a stabilizing effect on CTRD mRNA targets.
  • DXO1, another PAP-sensitive exoribonuclease, was identified as involved in CTRD, potentially targeting NAD+-capped mRNAs, particularly those related to stress responses.

Conclusions:

  • This study enhances the understanding of CTRD regulation in Arabidopsis.
  • The findings indicate that multiple exoribonucleases, beyond XRN4, can be involved in the CTRD pathway.
Keywords:
3ʹ-phosphoadenosine 5ʹ-phosphateArabidopsisCo-translational mRNA decayDXO1FRY1XRN4

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