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Updated: Aug 8, 2026

Patch Clamp Recording of Ion Channels Expressed in Xenopus Oocytes
Published on: October 16, 2008
Maturation of Xenopus oocytes is not accompanied by electrode-detectable calcium changes
Abstract:
Ca2+-sensitive microelectrodes were used to study changes in cytoplasmic free calcium during progesterone-induced meiotic maturation in Xenopus laevis oocytes. In contrast to previous reports, no changes were detected during the maturation process, although a large activation pulse of Ca2+ was seen when in vitro matured eggs were parthenogenetically activated. The resting level of Ca2+ in the oocytes was 0.14 microM +/- 0.05.
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