Effect of progesterone on canine colonic smooth muscle

This study was undertaken to ascertain the effect and mode of action of progesterone on canine colonic smooth muscle in vitro. Circularly orientated strips of smooth muscle exhibited low-amplitude contractions at the slow wave frequency. Longitudinally orientated strips exhibited larger-amplitude contractions that were associated, electrically, with a series of "spikes" superimposed on a high-frequency oscillation in membrane potential. Progesterone reduced the contractile force of both the circularly and longitudinally orientated strips and the contractile frequency of the longitudinally orientated strips in a dose-dependent manner; this inhibitory effect was observed in the presence of tetrodotoxin but not atropine. The stimulatory effects of a high-potassium superfusate were antagonized by progesterone and verapamil, the effect of progesterone being reversed by increasing the calcium concentration of the superfusate. We conclude that progesterone exerts an inhibitory effect on colonic smooth muscle and this may be mediated by changes in the cytoplasmic calcium concentration.