Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

SpyCEP dismantles neutrophil immunity via disorder-driven chemokine remodeling and GAG targeting.

Proceedings of the National Academy of Sciences of the United States of America·2026
Same author

Vaccination elicits HIV broadly neutralizing antibodies in primates.

Nature·2026
Same author

Translating Innovation to Clinic: End-to-End Bioprocess Development and cGMP Manufacturing of N332-GT5 HIV Vaccine Candidate for First-in-Human Trials HVTN144.

bioRxiv : the preprint server for biology·2026
Same author

Diverse germline-targeting HIV Env immunogens select for distinct mutations in the same knock-in mice B cell receptors.

Nature communications·2026
Same author

The Q226H Mutation in Avian H5N1 Hemagglutinin Mediates a Path towards Structural Adaptation in Humans.

bioRxiv : the preprint server for biology·2026
Same author

A long-chain heparan sulfate capture mechanism directs paracrine GDNF-GFRα1 signalling through RET.

bioRxiv : the preprint server for biology·2026

Related Experiment Video

Updated: May 27, 2025

Profiling Anti-Neu5Gc IgG in Human Sera with a Sialoglycan Microarray Assay
09:13

Profiling Anti-Neu5Gc IgG in Human Sera with a Sialoglycan Microarray Assay

Published on: July 13, 2017

9.4K

The minimum information required for a glycomics experiment (MIRAGE) project: improving the standards for reporting

Hiroaki Tateno1, Lara K Mahal2, Ten Feizi3

  • 1Cellular and Molecular Biotechnology Research Institute, National Institute of Advanced Industrial Science and Technology (AIST), Central 6, 1-1-1 Higashi, Tsukuba, Ibaraki 305-8566, Japan.

Glycobiology
|February 19, 2025
PubMed
Summary

The Minimum Information Required for a Glycomics Experiment (MIRAGE) project proposes new guidelines for lectin microarray data reporting. These standards aim to improve data interpretation and comparability in glycobiology research.

Keywords:
MIRAGEglycanglycomicsguidelinelectin microarrays

More Related Videos

Author Spotlight: Advancing Protein Glycosylation Research Using a Fully Automated System
05:19

Author Spotlight: Advancing Protein Glycosylation Research Using a Fully Automated System

Published on: June 28, 2024

739
Purification and Analytics of a Monoclonal Antibody from Chinese Hamster Ovary Cells Using an Automated Microbioreactor System
10:50

Purification and Analytics of a Monoclonal Antibody from Chinese Hamster Ovary Cells Using an Automated Microbioreactor System

Published on: May 1, 2019

14.5K

Related Experiment Videos

Last Updated: May 27, 2025

Profiling Anti-Neu5Gc IgG in Human Sera with a Sialoglycan Microarray Assay
09:13

Profiling Anti-Neu5Gc IgG in Human Sera with a Sialoglycan Microarray Assay

Published on: July 13, 2017

9.4K
Author Spotlight: Advancing Protein Glycosylation Research Using a Fully Automated System
05:19

Author Spotlight: Advancing Protein Glycosylation Research Using a Fully Automated System

Published on: June 28, 2024

739
Purification and Analytics of a Monoclonal Antibody from Chinese Hamster Ovary Cells Using an Automated Microbioreactor System
10:50

Purification and Analytics of a Monoclonal Antibody from Chinese Hamster Ovary Cells Using an Automated Microbioreactor System

Published on: May 1, 2019

14.5K

Area of Science:

  • Glycobiology
  • Glycoanalytics
  • Bioinformatics

Background:

  • Lectin microarrays are crucial for glycan profiling across biological samples.
  • Current reporting lacks sufficient detail on experimental methods like sample preparation and labeling.
  • Existing Minimum Information Required for a Glycomics Experiment (MIRAGE) guidelines cover other glycomic analyses.

Purpose of the Study:

  • To establish standardized reporting guidelines for lectin microarray experiments.
  • To ensure comprehensive documentation of experimental methods and data.
  • To enhance the reproducibility and comparability of lectin microarray studies.

Main Methods:

  • Development of guidelines based on the seven key areas of a lectin microarray workflow.
  • Recommendations for minimum information required for reporting results.
  • Expert consensus from glycobiology, glycoanalytics, and glycoinformatics specialists.

Main Results:

  • Proposed MIRAGE lectin microarray guidelines offer specific recommendations for reporting.
  • Guidelines cover essential aspects from sample preparation to data analysis.
  • The guidelines aim to standardize the reporting of lectin microarray data.

Conclusions:

  • Adoption of MIRAGE lectin microarray guidelines will improve data interpretation.
  • Standardized reporting will facilitate inter-laboratory data comparison.
  • These guidelines are expected to encourage deposition of lectin microarray data in public databases.