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A new technique for antioxidant walnut peptide preparation directly from walnut cake: Enzymatic preparation process
Jiahui Hong1, Yinzi Chang1, Hong Feng1
1College of Food and Health, Zhejiang A&F University, Hangzhou 311300, China; National Key Laboratory for Development and Utilization of Forest Food Resources, Zhejiang A & F University, Hangzhou 311300, China.
Abstract:
The lack of scalable production methods limits the commercial production viability of walnut peptides. To overcome this obstacle, enzyme membrane reactors (EMRs) were used to continuously produce bioactive peptides (called CEMR) directly from walnut cake. The optimum operating conditions were pH 10.7, an [E/S] ratio of 11 %, and a temperature of 44 °C, which resulted in a peptide yield of 256.0 ± 4.66 mg/g cake and a protein conversion degree reaching 63.49 ± 0.82 %. Kinetic analysis showed that affinity between alkaline protease and walnut cake can be enhanced by EMR (km decreased, kA increased). The antioxidant results showed that the strongest antioxidant activity was detected in CEMR. The composition of amino acids and molecular weight distribution results showed that the highest content of Glu (20.20 ± 0.48 %), Asp (20.70 ± 0.95 %), and peptides with molecular weight < 1KD (51.92 %) were detected in CEMR. The results of CEMR provide a new option for simplifying the production process of walnut peptide.
