Search research articles

ABOUT JoVE

Overview Leadership Blog JoVE Help Center

AUTHORS

Publishing Process Editorial Board Scope & Policies Peer Review FAQ Submit

LIBRARIANS

Testimonials Subscriptions Access Resources Library Advisory Board FAQ

RESEARCH

JoVE Journal Methods Collections JoVE Encyclopedia of Experiments Archive

EDUCATION

JoVE Core JoVE Business JoVE Science Education JoVE Lab Manual Faculty Resource Center Faculty Site

Terms & Conditions of Use

Related Concept Videos

Search research articles

Home
Research Domains
Agricultural, Veterinary And Food Sciences
Agricultural Biotechnology
Agricultural Biotechnology Diagnostics (incl. Biosensors)
Development And Validation Of A Point-of-care Molecular Assay For Sacbrood Virus (sbv) Diagnosis In Apiaries.

Home
Research Domains
Agricultural, Veterinary And Food Sciences
Agricultural Biotechnology
Agricultural Biotechnology Diagnostics (incl. Biosensors)
Development And Validation Of A Point-of-care Molecular Assay For Sacbrood Virus (sbv) Diagnosis In Apiaries.

Related Experiment Video

Development and Validation of a Quantitative PCR Method for Equid Herpesvirus-2 Diagnostics in Respiratory Fluids

Development and Validation of a Quantitative PCR Method for Equid Herpesvirus-2 Diagnostics in Respiratory Fluids

Published on: March 17, 2016

Development and validation of a point-of-care molecular assay for sacbrood virus (SBV) diagnosis in apiaries.

Juhaeng Heo¹, Doo-Sung Cheon², Nyun-Ki Chung³

¹Department of Veterinary Pathology, College of Veterinary Medicine, Seoul National University, 1 Gwanak-ro, Gwanak-gu, Seoul 08826, Republic of Korea.

Journal of Virological Methods

|February 19, 2025

View abstract on PubMed

Summary

This summary is machine-generated.

A new diagnostic kit for Sacbrood virus (SBV) offers accurate, on-site detection for honeybee health. This point-of-care tool aids beekeepers in managing SBV, protecting vital pollinators and apiculture.

Keywords:

Honeybee Point-of-care (POC) diagnostics RT-qPCR Sacbrood virus (SBV)

More Related Videos

High-throughput Detection of Respiratory Pathogens in Animal Specimens by Nanoscale PCR

High-throughput Detection of Respiratory Pathogens in Animal Specimens by Nanoscale PCR

Published on: November 28, 2016

Using a Pan-Viral Microarray Assay Virochip to Screen Clinical Samples for Viral Pathogens

Using a Pan-Viral Microarray Assay Virochip to Screen Clinical Samples for Viral Pathogens

Published on: April 27, 2011

Related Experiment Videos

Development and Validation of a Quantitative PCR Method for Equid Herpesvirus-2 Diagnostics in Respiratory Fluids

Development and Validation of a Quantitative PCR Method for Equid Herpesvirus-2 Diagnostics in Respiratory Fluids

Published on: March 17, 2016

High-throughput Detection of Respiratory Pathogens in Animal Specimens by Nanoscale PCR

High-throughput Detection of Respiratory Pathogens in Animal Specimens by Nanoscale PCR

Published on: November 28, 2016

Using a Pan-Viral Microarray Assay Virochip to Screen Clinical Samples for Viral Pathogens

Using a Pan-Viral Microarray Assay Virochip to Screen Clinical Samples for Viral Pathogens

Published on: April 27, 2011

Area of Science:

Veterinary Entomology
Molecular Diagnostics
Apiculture Science

Background:

Sacbrood virus (SBV) poses a significant threat to honeybee populations, causing considerable economic damage to the apiculture industry.
Traditional diagnostic methods like RT-qPCR are accurate but impractical for field use in apiaries.

Purpose of the Study:

To develop and evaluate the XQ SBV Dx Kit for point-of-care (POC) on-site molecular diagnosis of SBV using the XQ Station device.
To compare the diagnostic performance of the XQ SBV Dx Kit with standard laboratory-based RT-qPCR.

Main Methods:

Development of the XQ SBV Dx Kit integrated with the XQ Station POC RT-qPCR device for automated nucleic acid extraction, amplification, and detection.
Assessment of diagnostic performance using clinical SBV-infected honeybee samples, including limit of detection (LOD) and specificity testing against 11 other honeybee pathogens.

Clinical evaluation of the kit's sensitivity and specificity in field conditions.

Main Results:

The XQ SBV Dx Kit demonstrated a comparable LOD of 10^2 copies/reaction to standard RT-qPCR.
The kit showed superior sensitivity, detecting 83.3% of replicates at 10^1 copies/reaction versus 58.3% for RT-qPCR.
High clinical sensitivity (98.4%) and specificity (97.0%) were achieved, with no cross-reactivity observed against other honeybee pathogens.

Conclusions:

The XQ SBV Dx Kit provides a reliable, sensitive, and specific tool for on-site SBV diagnosis in apiaries.
This POC diagnostic solution is crucial for timely honeybee health management and supports sustainable apicultural practices.
The XQ SBV Dx Kit represents a significant advancement for disease surveillance and control in honeybee populations.