Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Glial Cells01:04

Glial Cells

Overview
Neural Regulation01:37

Neural Regulation

Digestion begins with a cephalic phase that prepares the digestive system to receive food. When our brain processes visual or olfactory information about food, it triggers impulses in the cranial nerves innervating the salivary glands and stomach to prepare for food.
Cerebral Hemispheres01:05

Cerebral Hemispheres

The human brain, a complex organ, is functionally divided into two cerebral hemispheres—left and right. These hemispheres are interconnected by a structure of paramount importance, the corpus callosum. This substantial bundle of neural fibers is not just a bridge between the hemispheres but a crucial element for the brain's comprehensive functioning. It enables efficient communication between the two hemispheres, allowing each side of the brain to control and receive sensory and motor...
Nervous Tissue: Neuron Types01:19

Nervous Tissue: Neuron Types

Neurons, the fundamental units of the nervous system, can be classified based on both their structural and functional characteristics.
Structurally, neurons are categorized into three main types: multipolar, bipolar, and unipolar (or pseudounipolar). Multipolar neurons, which are the most common type in the brain and spinal cord, as well as all motor neurons, possess multiple dendrites and a single axon.
Bipolar neurons, on the other hand, have one primary dendrite and one axon. They are...
Lateralization01:28

Lateralization

Brain lateralization refers to the division of mental processes and functions between the two hemispheres of the brain, a phenomenon that optimizes neural efficiency and underpins complex abilities in humans. This specialization allows each hemisphere to perform tasks where it has a comparative advantage, facilitating more refined cognitive capabilities across different domains.
Functional Divisions of the Nervous System01:23

Functional Divisions of the Nervous System

The nervous system, responsible for sensing, integrating, and responding to various stimuli, is divided into the central nervous system (CNS) and the peripheral nervous system (PNS). The PNS has two functional divisions: the sensory or afferent division and the motor or efferent division.
The sensory division transmits information from sensory receptors in the body to the CNS. It provides the CNS with knowledge about somatic senses (such as tactile, thermal, pain, and proprioceptive sensations)...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Annotation of glycoside hydrolases in unassembled metagenomes using CAZyO<sub>GH</sub>.

Bioinformatics advances·2026
Same author

ez-CAZy a reference annotation database for linking glycoside hydrolase sequence to enzymatic activity.

Scientific reports·2025
Same author

Warming is Associated With More Encoded Antimicrobial Resistance Genes and Transcriptions Within Five Drug Classes in Soil Bacteria: A Case Study and Synthesis.

Environmental microbiology·2025
Same author

A truncated isoform of Connexin43 caps actin to organize forward delivery of full-length Connexin43.

The Journal of cell biology·2024
Same author

Actin Isoform Composition and Binding Factors Fine-Tune Regulatory Impact of Mical Enzymes.

International journal of molecular sciences·2023
Same author

Mapping Molecular Interaction Interface Between Diaphanous Formin-2 and Neuron-Specific Drebrin A.

Journal of molecular biology·2023

Related Experiment Video

Updated: Jun 16, 2026

Easy and Reproducible Low-Density Primary Culture using Frozen Stock of Embryonic Hippocampal Neurons
04:26

Easy and Reproducible Low-Density Primary Culture using Frozen Stock of Embryonic Hippocampal Neurons

Published on: January 27, 2023

2.3K

Functional Differences Between Neuronal and Non-neuronal Isoforms of Drebrin.

Sargis Srapyan1, Mikayel Mkrtchyan1, Renaud Berlemont2

  • 1Department of Chemistry and Biochemistry, California State University, Long Beach (CSULB), Long Beach, CA 90840, USA.

Journal of Molecular Biology
|February 19, 2025
PubMed
Summary

Neuronal drebrin A and embryonic drebrin E have distinct functions in stabilizing actin filaments. Drebrin A exhibits stronger actin capping and resistance to severing, crucial for neuronal development.

Keywords:
actincofilindrebrin Adrebrin Eneuronal

More Related Videos

Assay for Blood-brain Barrier Integrity in Drosophila melanogaster
09:08

Assay for Blood-brain Barrier Integrity in Drosophila melanogaster

Published on: September 18, 2019

8.2K
DiOLISTIC Labeling of Neurons from Rodent and Non-human Primate Brain Slices
09:21

DiOLISTIC Labeling of Neurons from Rodent and Non-human Primate Brain Slices

Published on: July 6, 2010

23.9K

Related Experiment Videos

Last Updated: Jun 16, 2026

Easy and Reproducible Low-Density Primary Culture using Frozen Stock of Embryonic Hippocampal Neurons
04:26

Easy and Reproducible Low-Density Primary Culture using Frozen Stock of Embryonic Hippocampal Neurons

Published on: January 27, 2023

2.3K
Assay for Blood-brain Barrier Integrity in Drosophila melanogaster
09:08

Assay for Blood-brain Barrier Integrity in Drosophila melanogaster

Published on: September 18, 2019

8.2K
DiOLISTIC Labeling of Neurons from Rodent and Non-human Primate Brain Slices
09:21

DiOLISTIC Labeling of Neurons from Rodent and Non-human Primate Brain Slices

Published on: July 6, 2010

23.9K

Area of Science:

  • Neuroscience
  • Cell Biology
  • Biochemistry

Background:

  • The actin cytoskeleton is essential for neuronal function.
  • Drebrin is a key F-actin binding protein involved in filament stabilization.
  • The switch from non-neuronal drebrin E to neuron-specific drebrin A during brain development is not fully understood.

Purpose of the Study:

  • To investigate the functional differences between drebrin A and drebrin E isoforms.
  • To elucidate the molecular mechanisms underlying these functional distinctions.
  • To understand the evolutionary significance of drebrin isoform switching.

Main Methods:

  • Mutagenesis
  • Bulk solution assays
  • Time-lapse total internal reflection fluorescence (TIRF) microscopy

Main Results:

  • Drebrin A and E are functionally distinct in inhibiting F-actin depolymerization.
  • Both isoforms cap the barbed end of actin filaments, but drebrin A shows significantly stronger capping activity.
  • The adult-specific exon in drebrin A contains an actin-binding interface enhancing its capping.
  • F-actin decorated by drebrin A is more resistant to cofilin-mediated severing than that decorated by drebrin E.

Conclusions:

  • Novel molecular insights into the functional differences between drebrin isoforms.
  • Enhanced understanding of cytoskeletal regulation in neurons.
  • Provides a basis for interpreting data on drebrin isoform silencing or knockout.