Ammonia transformation by potential consortium in the aquatic system

In the present research, the removal of high concentration of ammonia, nitrite and nitrate was determined by using the efficient ammonia-oxidizing bacterial isolates (AOB AMS 1, AOB AMS 2, AOB AMS 5 and AOB AMSS 1) along with the consortium of all four bacterial isolates from the aquaculture both in lab-scale as well as in-situ at bioreactor level. It was found that the highest percentage of ammonia was degrade by AOB AMS 2 (99.64%± 5) followed by AOB AMS 5 (99.51%± 4.6), AOB AMSS 1 (99.38%± 5) and AOB AMS 1 (89.66%± 3.5) from an initial concentration of 30 mg/L whereas from an initial concentration of 60 mg/L, the removal rate of each strain was AOB AMS 5 (99.36%± 5), AOB AMSS 1 (99.15%± 5.60), AOB AMS 2 (98.78%± 5.3) and AOB AMS 1 (88.36%± 3.2) and their consortium removes (99.97%± 5.20)at 60 mg/L. Further, in-situ transformation was carried out in the river water with 5% of inoculum. Their growth was monitored (initial and final) by observing the colony forming unit (CFU) and transformation of ammonia and nitrite was also observed and it was found that (100 ± 2%)of ammonia was removed. The growth of consortium was 3 × 10²CFU/ml on 1st day, 2.5 × 10⁴ CFU/ml on 5th day and 4.2 × 10⁵ CFU/ml on 10th day. This study proved that the bacterial consortium of AOB AMS 1, AOB AMS 2, AOB AMS 5 and AOB AMSS 1 was highly efficient for the removal of ammonia, nitrite and nitrate from the aquatic system to reduce the toxic level from aquaculture environment.Currently we observed the transformation efficiency at lab-scaleand in-situ level.