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Updated: May 27, 2025
Digital PCR for Quantifying Circulating MicroRNAs in Acute Myocardial Infarction and Cardiovascular Disease
Published on: July 3, 2018
The association of serum hsa-miR-21-5p expression with the severity and prognosis of heart failure with reduced
Lingmiao Wang1, Ailin Guo2, Shuang Liang3
1Department of Anesthesiology, Xiang'an Hospital of Xiamen University, School of Medicine, Xiamen University, No.2000 Xiang'an East Road, Xiamen, 361101, China.
Insights
Serum hsa-miR-21-5p levels are higher in heart failure with reduced ejection fraction (HFrEF) patients and correlate with disease severity. Elevated hsa-miR-21-5p predicts cardiovascular events, offering a new prognostic marker.
Area of Science:
- Biochemistry
- Cardiology
- Molecular Biology
Background:
- Heart failure with reduced ejection fraction (HFrEF) affects millions globally.
- Identifying reliable biomarkers for HFrEF severity and prognosis is crucial.
Purpose of the Study:
- To investigate the association between serum hsa-miR-21-5p expression and HFrEF severity.
- To evaluate the predictive value of hsa-miR-21-5p for cardiovascular outcomes in HFrEF patients.
Main Methods:
- Retrospective analysis of 105 HFrEF patients and 86 controls.
- RT-qPCR for serum hsa-miR-21-5p detection.
- Correlation, ROC, Kaplan-Meier, and COX regression analyses.
Main Results:
- HFrEF patients exhibited higher serum hsa-miR-21-5p levels compared to controls.
- hsa-miR-21-5p expression correlated with cardiac function and hemodynamic indicators.
- Elevated hsa-miR-21-5p predicted cardiovascular re-hospitalization and death, identified as an independent risk factor.
Conclusions:
- Serum hsa-miR-21-5p is a potential biomarker for HFrEF severity.
- hsa-miR-21-5p may serve as a valuable prognostic indicator for cardiovascular events in HFrEF.
Background:
Heart failure poses a huge burden on the global population, and approximately 50% cases have reduced ejection fraction, namely, heart failure with reduced ejection fraction (HFrEF). The research was supposed to explore the relationship between serum hsa-miR-21-5p expression and the severity/prognosis of HFrEF.
Methods:
A retrospective analysis was conducted on 105 HFrEF patients and 86 healthy volunteers. RT-qPCR was used for the detection of serum hsa-miR-21-5p levels. Whether serum hsa-miR-21-5p expression correlated to cardiac function and hemodynamic indicators was evaluated using Spearman correlation analysis. Receiver operating characteristic and Kaplan-Meier analyses were implemented to evaluate the predictive significance of serum hsa-miR-21-5p for cardiovascular re-hospitalization and cardiovascular deaths in HFrEF patients. Independent risk factors for cardiovascular deaths were analyzed based on COX regression.
Results:
Higher heart rate, fasting plasma glucose (FPG), N-terminal pro-brain natriuretic peptide (NT-pro-BNP), left atrial diameter (LAD), systolic pulmonary artery pressure (sPAP), pulmonary vascular resistance (PVR), pulmonary capillary wedge pressure (PCWP), and hsa-miR-21-5p as well as lower left ventricular ejection fraction (LVEF) and left ventricular fractional shortening (LVFS) were detected in the HFrEF patients than in the controls. Hsa-miR-21-5p expression increased in HFrEF patients with the increase of NYHA grade. Serum hsa-miR-21-5p expression in HFrEF patients was positively relevant to NT-pro-BNP, LAD, sPAP, PVR, and PCWP but negatively related to LVEF and LVFS. Serum hsa-miR-21-5p had a high predictive value for cardiovascular re-hospitalization and cardiovascular deaths in HFrEF patients. High hsa-miR-21-5p expression was an independent dangerous factor for cardiovascular deaths.
Conclusion:
Collectively, hsa-miR-21-5p expression in HFrEF patients is correlated with disease severity and the risk of cardiovascular re-hospitalization and cardiovascular deaths in HFrEF patients, indicating a new direction for evaluating the disease situation and prognosis.
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