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High-Performance Liquid Chromatography: Types of Detectors01:15

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The role of the detectors in High-Performance Liquid Chromatography (HPLC) is to analyze the solutes as they exit from the chromatographic column. The detector recognizes the solute's property and generates corresponding electrical signals, which are converted into a readable graph of the detector's response versus elution time called a chromatogram at the computer. There are several types of HPLC detectors, each with its own advantages and limitations, depending on the analyte...
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Continuous High-Throughput Plasma Separation for Blood Biomarker Sensing Using a Hydrodynamic Microfluidic Device.

Hesam Abouali1, Fatemeh Keyvani1, Seied Ali Hosseini1

  • 1Department of Electrical and Computer Engineering, University of Waterloo, Waterloo, ON, N2L 3G1, Canada.

Advanced Healthcare Materials
|February 20, 2025
PubMed
Summary

A new microfluidic device offers continuous, high-throughput blood plasma separation. This Hydrodynamic Continuous, High-Throughput Plasma Separator (HCHPS) achieves high purity and yield for biomarker detection.

Keywords:
aptamer biosensorautomated analysisbiomarkerblood plasma separationmicrofluidic

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Area of Science:

  • Biomedical Engineering
  • Fluid Dynamics
  • Clinical Diagnostics

Background:

  • Effective blood plasma separation is crucial for clinical biomarker detection.
  • Existing methods like centrifugation and current microfluidics have limitations in throughput, cost, yield, and purity.
  • There is a need for continuous, high-throughput plasma separation technologies meeting clinical demands.

Purpose of the Study:

  • To develop and validate a microfluidic device for continuous, cost-effective, high-throughput blood plasma separation.
  • To optimize microchannel design for improved plasma extraction yield and purity.
  • To confirm the suitability of the separated plasma for downstream biomarker analysis.

Main Methods:

  • Design and fabrication of a microfluidic device utilizing passive hydrodynamic principles.
  • Computational and experimental assessments to optimize side channel lengths for plasma extraction.
  • Testing the device with whole blood and diluted blood, evaluating purity, yield, and hemolysis.
  • Validation of plasma quality using bead-based fluorescence and electrochemical aptamer biosensing.

Main Results:

  • The developed Hydrodynamic Continuous, High-Throughput Plasma Separator (HCHPS) device achieves 47-64% purity and 10-18% yield for whole blood, with reduced hemolysis compared to centrifugation.
  • Separation of diluted blood yielded 62-97% purity with comparable yield.
  • Demonstrated successful downstream biomarker analysis using the separated plasma via fluorescence and electrochemical biosensing.

Conclusions:

  • The HCHPS microfluidic device provides an efficient solution for continuous, high-throughput plasma separation.
  • The optimized design ensures admissible yield and purity, suitable for clinical biomarker detection.
  • This technology offers a promising alternative to conventional methods for preparing plasma samples.