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Updated: May 27, 2025
Stereotaxic Surgery for Implantation of Microelectrode Arrays in the Common Marmoset Callithrix jacchus
Published on: September 29, 2019
Determination of Minimum Alveolar Concentrations of Isoflurane and Effective Plasma Concentration of Propofol in
Kanako Muta1,2, Makoto Koizumi3, Takako Miyabe-Nishiwaki4
1Laboratory of Veterinary Surgery, Graduate School of Agricultural and Life Sciences, The University of Tokyo, Tokyo, Japan.
Background:
Common marmosets (Callithrix jacchus) have been widely used as laboratory animals; However, limited sedation and anesthetic protocols have yet to be established. In this study, the minimum alveolar concentration of an inhalant (isoflurane) and effective predicted plasma concentration of an injectable anesthetic (propofol) were measured for optimization of sedation and anesthetic protocols in marmosets.
Methods:
The minimum alveolar concentrations (MACs) for several stimulations (nociceptive stimulation, endotracheal intubation, and non-painful direct stimulation), MAC-noci, MAC-extb, and MAC-awake, respectively, were measured for isoflurane with the up-and-down method from four healthy marmosets. Predicted plasma concentrations 50 (Cp50s), which are equivalent to MACs of isoflurane, Cp50-noci, Cp50-extb, and Cp50-awake, respectively, were measured for propofol.
Results:
MAC-noci and MAC-extb of isoflurane in marmosets were 1.91% and 1.38%, respectively. MAC-awake was not determined owing to technical difficulties. Cp50-noci, Cp50-extb, and Cp50-awake were 9.45, 7.21, and 3.54 μg/mL, respectively.
Conclusions:
The obtained results refined existing isoflurane and propofol for sedation and anesthesia in marmosets.
