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Preparation of Adipose Progenitor Cells from Mouse Epididymal Adipose Tissues
Published on: August 25, 2020
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Adipose-derived stem cells exosomal circHIPK3 protects ovarian function by regulating MAPK signaling
Wei Zhao1,2, Da Erhan3, Shujun Liu4
1Department of Reproductive Center, Xilingol League Central Hospital, Inner Mongolia Xilingol League, China.
Indian Journal of Pharmacology
|February 20, 2025
Summary
Adipose-derived stem cell exosomes carrying circHIPK3 improve ovarian aging in mice by enhancing granulosa cell viability and function. Depleting circHIPK3 reversed these beneficial effects.
Area of Science:
- Stem cell biology
- Extracellular vesicles
- Reproductive medicine
Background:
- Adipose-derived stem cells (ADSCs) and their exosomes show therapeutic promise.
- Exosomes transport bioactive molecules, including circular RNAs (circRNAs).
- circRNAs are enriched in exosomes and play regulatory roles.
Purpose of the Study:
- To investigate the role of circHIPK3 delivered by ADSC-derived exosomes in ovarian aging.
- To determine the impact on primary granulosa cells (pGCs) and overall ovarian function.
Main Methods:
- Isolation and characterization of ADSC-derived exosomes.
- Treatment of aging mice and pGCs with exosomes containing circHIPK3 or its siRNA.
- Assessment of ovarian morphology, follicular development, cell senescence, and apoptosis.
- Analysis of p38 protein ubiquitination.
Main Results:
- ADSC-exosomes successfully delivered circHIPK3 to pGCs.
- Exosomal circHIPK3 treatment improved ovarian weight and follicular development in aged mice.
- circHIPK3 depletion exacerbated apoptosis and senescence in pGCs and ovaries.
- circHIPK3 modulated p38 ubiquitination, promoting pGC viability.
Conclusions:
- Exosomal circHIPK3 is a key mediator in improving ovarian function in aging mice.
- circHIPK3 delivered via ADSC-exosomes enhances pGC viability and function.
- Modulating circHIPK3 levels offers a potential therapeutic strategy for ovarian aging.
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