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In vitro Transcription and Capping of Gaussia Luciferase mRNA Followed by HeLa Cell Transfection
Published on: March 26, 2012
CleanCap M6 inhibits decapping of exogenously delivered IVT mRNA
Zachary F Mandell1, Andrew Ujita2, Jordana Henderson2
1RNA Innovation Center, Institute for NanoBioTechnology, Johns Hopkins University, Baltimore, MD 21218, USA.
None:
Co-transcriptional capping allows exogenous mRNAs to yield robust protein expression. Identifying additional mRNA modifications that further boost protein output will be crucial for developing more efficacious mRNA therapies. Using in vitro approaches, we found that the co-transcriptional cap analog m7G3'OMepppm6A2'OMepG, CleanCap M6, resists enzymatic decapping and that this decreased susceptibility to decapping correlated with substantially increased protein expression in vivo compared to mRNAs capped using existing industry standards.
