Related Experiment Video
Updated: Jun 22, 2026

Formulations for Freeze-drying of Bacteria and Their Influence on Cell Survival
Published on: August 3, 2013
Freeze drying microencapsulation using whey protein, maltodextrin and corn powder improved survivability of
Benjamaporn Poopan1,2, Anongnard Kasorn3, Narathip Puttarat2
1Molecular Biology Program, Faculty of Medicine, Srinakharinwirot University, 114 Sukhumvit 23, Wattana District, Bangkok, 10110 Thailand.
Abstract:
Various studies demonstrated that probiotics play important roles in maintaining the balance of microorganisms in the body. Some strains produce bile salt hydrolase enzyme (BSH), which is an indirect mechanism for lowering cholesterol. BSH-producing probiotics as a supplement might be an alternative way to help reducing cholesterol in the body. The aim of this study was to investigate the effects of different microcapsule formulations with selected vegetable powders on growth characteristics of 3 Thai probiotic strains, Lactobacillus gasseri TM1, Lacticaseibacillus rhamnosus TM7, and L. rhamnosus TM14. Probiotics were cultured in MRS broth supplemented with 5 vegetable powders. Corn powder significantly increased growth rate of probiotics from 109 to 1012 CFU/ml. Therefore, different microcapsule formulations by Maillard reaction of whey protein isolate and maltodextrin mixed with and without corn powder were studied. The results showed that probiotic microcapsules formulated with corn powder significantly effectively sustained probiotic viability under gastrointestinal and storage conditions.
Supplementary Information:
The online version contains supplementary material available at 10.1007/s10068-024-01706-w.
Related Concept Videos
Endospores and Sporulation
Microbes in Food Production
Microbes in the Production of Fermented Foods
Pasteurization and Food Preservation
Principles of Food Preservation
Methods of Controlling Food Spoilage

