Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Alternative RNA Splicing02:18

Alternative RNA Splicing

20.9K
Alternative RNA splicing is the regulated splicing of exons and introns to produce different mature mRNAs from a single pre-mRNA. Unlike in constitutive splicing where a single gene produces a single type of mRNA, alternative splicing allows an organism to produce multiple proteins from a single gene and plays an important role in protein diversity.
There are five types of alternative RNA splicing that vary in the ways the pre-mRNA segments are removed or retained in the mature mRNA. The first...
20.9K
RNA Splicing01:32

RNA Splicing

55.9K
Splicing is the process by which eukaryotic RNA is edited before its translation into protein. The RNA strand transcribed from eukaryotic DNA is called the primary transcript. The primary transcripts that become mRNAs are called precursor messenger RNAs (pre-mRNAs). Eukaryotic pre-mRNA contains alternating sequences of exons and introns. Exons are nucleotide sequences that code for proteins, whereas introns are the non-coding regions. In RNA splicing, introns are removed and exons are bonded...
55.9K
Chromatin Structure Regulates pre-mRNA Processing02:41

Chromatin Structure Regulates pre-mRNA Processing

6.9K
In eukaryotic cells, nascent mRNA transcripts need to undergo many post-transcriptional modifications to reach the cell cytoplasm and translate into functional proteins. For a long time, transcription and pre-mRNA processing were considered two independent events that occur sequentially in the cell. However, it has now been well established that transcription and pre-mRNA processing are two simultaneous processes that are precisely regulated inside the cell.
The chromatin structure, especially...
6.9K
Regulation of Expression at Multiple Steps01:23

Regulation of Expression at Multiple Steps

864
The gene expression in cells is regulated at different stages: (i) transcription, (ii) RNA processing, (iii) RNA localization, and (iv) translation. Transcriptional regulation is mediated by regulatory proteins such as transcription factors, activators, or repressors—these control gene expression by initiating or inhibiting the transcription of genes. Once a precursor or pre-mRNA is produced, it undergoes post-transcriptional modification, including 5' capping, splicing, and the...
864
Pre-mRNA Processing: RNA Splicing01:36

Pre-mRNA Processing: RNA Splicing

5.2K
5.2K
Chromatin Structure and RNA Splicing02:41

Chromatin Structure and RNA Splicing

2.7K
2.7K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Orai1 is required for Ca<sup>2+</sup>-dependent plasma membrane repair and mechanoadaptation.

bioRxiv : the preprint server for biology·2026
Same author

Predictive modeling of signal-responsive cis-elements in human red blood cell precursors.

Nucleic acids research·2026
Same author

Kilobase-scale compartments enabled by CRUSH reveal regulatory programs across cell types, single-cells, and ancient mammoths.

bioRxiv : the preprint server for biology·2026
Same author

Multi-site DMS probing reveals higher-order structure of RNA-protein complexes in living cells.

Molecular cell·2026
Same author

Endogenous promoter G-quadruplexes scaffold apurinic/apyrimidinic endonuclease (APE1) to drive gene expression.

Nucleic acids research·2026
Same author

MOTLAB: A Weighted Multi-Omics Transfer Learning Approach to Mitigate Breast Cancer Racial Disparities.

bioRxiv : the preprint server for biology·2026

Related Experiment Video

Updated: May 27, 2025

Using the E1A Minigene Tool to Study mRNA Splicing Changes
10:25

Using the E1A Minigene Tool to Study mRNA Splicing Changes

Published on: April 22, 2021

4.8K

ECD functions as a novel RNA-binding protein to regulate mRNA splicing.

Mohsin Raza, Asher Rajkumar Rajan, Achyuth Kalluchi

    Biorxiv : the Preprint Server for Biology
    |February 20, 2025
    PubMed
    Summary

    The human ecdysoneless protein (ECD) directly binds RNA, regulating cell cycle and survival. This RNA binding is crucial for its function in mRNA splicing and maintaining U5 snRNP complex expression.

    More Related Videos

    A Reporter Based Cellular Assay for Monitoring Splicing Efficiency
    08:53

    A Reporter Based Cellular Assay for Monitoring Splicing Efficiency

    Published on: September 15, 2021

    2.7K
    Engineering Artificial Factors to Specifically Manipulate Alternative Splicing in Human Cells
    10:06

    Engineering Artificial Factors to Specifically Manipulate Alternative Splicing in Human Cells

    Published on: April 26, 2017

    8.9K

    Related Experiment Videos

    Last Updated: May 27, 2025

    Using the E1A Minigene Tool to Study mRNA Splicing Changes
    10:25

    Using the E1A Minigene Tool to Study mRNA Splicing Changes

    Published on: April 22, 2021

    4.8K
    A Reporter Based Cellular Assay for Monitoring Splicing Efficiency
    08:53

    A Reporter Based Cellular Assay for Monitoring Splicing Efficiency

    Published on: September 15, 2021

    2.7K
    Engineering Artificial Factors to Specifically Manipulate Alternative Splicing in Human Cells
    10:06

    Engineering Artificial Factors to Specifically Manipulate Alternative Splicing in Human Cells

    Published on: April 26, 2017

    8.9K

    Area of Science:

    • Molecular Biology
    • Cell Biology

    Background:

    • The human ecdysoneless protein (ECD) is vital for cell cycle regulation and survival.
    • ECD's role in RNA splicing has been suggested through its association with splicing complexes.

    Purpose of the Study:

    • To investigate the direct RNA binding capabilities of ECD.
    • To elucidate the mechanism by which ECD influences RNA splicing and cell function.

    Main Methods:

    • Electrophoretic mobility shift assay and mutational analysis to determine RNA binding sites.
    • Enhanced CLIP-seq and RNA-seq to identify ECD-bound mRNAs and splicing aberrations.
    • Analysis of ECD's interaction with U5 small nuclear RNA (snRNA) and U5 small nuclear ribonucleoprotein (snRNP) components.

    Main Results:

    • ECD directly binds RNA via its N-terminal region (amino acids 135-148).
    • ECD depletion causes widespread RNA splicing defects and altered gene expression.
    • ECD directly binds U5 snRNA, essential for U5 snRNP component expression and cell proliferation.

    Conclusions:

    • ECD regulates RNA splicing through direct RNA association.
    • ECD's RNA binding activity is essential for its cellular functions, including maintaining U5 snRNP integrity and cell proliferation.