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SHINE: SERS-based Hepatotoxicity detection using Inference from Nanoscale Extracellular vesicle content.

Ugur Parlatan1, Luke Boudreau2,3, Hulya Torun1

  • 1Bio-Acoustic MEMS in Medicine (BAMM) Lab, Canary Center at Stanford, Department of Radiology, School of Medicine, Stanford University, California, CA, 94304, USA.

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Summary
This summary is machine-generated.

This study introduces a rapid, label-free surface-enhanced Raman spectroscopy (SERS) method for analyzing extracellular vesicles (EVs). The technique detects molecular changes in EVs, enabling early identification of drug-induced liver injury (hepatotoxicity).

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Area of Science:

  • Biomedical Engineering
  • Nanotechnology
  • Spectroscopy

Background:

  • Extracellular vesicles (EVs) are vital for diagnostics and therapeutics but challenging to characterize at the nanoscale.
  • Detecting drug-induced liver injury (hepatotoxicity) via EV molecular content is an emerging, unexplored area.

Purpose of the Study:

  • To develop a rapid, label-free surface-enhanced Raman spectroscopy (SERS) platform for analyzing EV molecular content.
  • To investigate the potential of this platform for detecting drug-induced hepatotoxicity using EV signatures.

Main Methods:

  • A novel SERS spectroscopy approach was employed for EV analysis.
  • The platform requires minimal sample volume (1.3 microliters) and provides results in under ten minutes.
  • Hepatic cell cultures were used to model acetaminophen-induced hepatotoxicity.

Main Results:

  • The platform successfully captured distinct and reproducible EV molecular changes in response to hepatotoxicity.
  • High accuracy was achieved, with root mean squared error as low as 3.80%.
  • Strong correlations were established between EV spectral data and conventional toxicity biomarkers, revealing drug-response signatures.

Conclusions:

  • Extracellular vesicles serve as dynamic reporters of cellular drug responses.
  • SERS-based EV detection offers a promising method for identifying and monitoring drug-induced hepatotoxicity.