Search research articles

ABOUT JoVE

Overview Leadership Blog JoVE Help Center

AUTHORS

Publishing Process Editorial Board Scope & Policies Peer Review FAQ Submit

LIBRARIANS

Testimonials Subscriptions Access Resources Library Advisory Board FAQ

RESEARCH

JoVE Journal Methods Collections JoVE Encyclopedia of Experiments Archive

EDUCATION

JoVE Core JoVE Business JoVE Science Education JoVE Lab Manual Faculty Resource Center Faculty Site

Terms & Conditions of Use

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by

Same author

Human interferon stimulated genes target ancient features of animal and bacterial viral replication.

bioRxiv : the preprint server for biology·2026

Same author

Egyptian rousette bat humoral immunity to H9 influenza hemagglutinin.

bioRxiv : the preprint server for biology·2026

Same author

Mechanistic and antigenic boundaries of Henipavirus and Parahenipavirus glycoproteins.

Nature communications·2026

Same author

Single-cell transcriptomics reveal altered B cell responses and T cell senescence in geriatric non-responders to COVID-19 mRNA vaccination.

iScience·2026

Same author

Gene syntax defines supercoiling-mediated transcriptional feedback.

Science (New York, N.Y.)·2026

Same author

The adaptive immune receptors in a big data world.

ImmunoHorizons·2026

Same journal

Genetic Impacts on Variability of Body Fat Distribution Uncover Gene-Environment and Gene-Gene Interactions.

bioRxiv : the preprint server for biology·2026

Same journal

16S ribosomal RNA modification drives transcript-specific translation efficiency.

bioRxiv : the preprint server for biology·2026

Same journal

FlcE latches onto the FliL-stator complex to turbocharge flagellar motility in <i>Borrelia burgdorferi</i>.

bioRxiv : the preprint server for biology·2026

Same journal

Synaptic pruning, myelination and the emergence of psychiatric disorders in late adolescence.

bioRxiv : the preprint server for biology·2026

Same journal

Structural and functional insights into the Rcs phosphorelay.

bioRxiv : the preprint server for biology·2026

Same journal

The structural basis of RanGAP1 regulation and catalysis in nuclear transport.

bioRxiv : the preprint server for biology·2026

See all related articles

Search research articles

Related Experiment Video

Updated: May 27, 2025

Detection of SARS-CoV-2 Neutralizing Antibodies using High-Throughput Fluorescent Imaging of Pseudovirus Infection

Detection of SARS-CoV-2 Neutralizing Antibodies using High-Throughput Fluorescent Imaging of Pseudovirus Infection

Published on: June 5, 2021

High-Throughput Antibody Neutralization Screening in Massively Parallel Droplet Arrays.

Matias Gutierrez-Gonzalez, Ahmed S Fahad, Cyrille L Delley

Biorxiv : the Preprint Server for Biology

|February 20, 2025

Summary

This summary is machine-generated.

Researchers developed a new method, Droplet Reporter Cell Testing for Neutralization (DrReCT-Neutralization), to efficiently discover neutralizing antibodies. This scalable platform enables faster identification of antibodies crucial for combating viral diseases.

More Related Videos

A Rapid, Multiplex Dual Reporter IgG and IgM SARS-CoV-2 Neutralization Assay for a Multiplexed Bead-Based Flow Analysis System

A Rapid, Multiplex Dual Reporter IgG and IgM SARS-CoV-2 Neutralization Assay for a Multiplexed Bead-Based Flow Analysis System

Published on: April 6, 2021

High Throughput Sequential ELISA for Validation of Biomarkers of Acute Graft-Versus-Host Disease

High Throughput Sequential ELISA for Validation of Biomarkers of Acute Graft-Versus-Host Disease

Published on: October 31, 2012

Related Experiment Videos

Last Updated: May 27, 2025

Detection of SARS-CoV-2 Neutralizing Antibodies using High-Throughput Fluorescent Imaging of Pseudovirus Infection

Detection of SARS-CoV-2 Neutralizing Antibodies using High-Throughput Fluorescent Imaging of Pseudovirus Infection

Published on: June 5, 2021

A Rapid, Multiplex Dual Reporter IgG and IgM SARS-CoV-2 Neutralization Assay for a Multiplexed Bead-Based Flow Analysis System

A Rapid, Multiplex Dual Reporter IgG and IgM SARS-CoV-2 Neutralization Assay for a Multiplexed Bead-Based Flow Analysis System

Published on: April 6, 2021

High Throughput Sequential ELISA for Validation of Biomarkers of Acute Graft-Versus-Host Disease

High Throughput Sequential ELISA for Validation of Biomarkers of Acute Graft-Versus-Host Disease

Published on: October 31, 2012

Area of Science:

Immunology
Biotechnology
Virology

Background:

Neutralizing antibodies are key for rapid immune defense against infections.
Current methods for discovering neutralizing antibodies at scale are limited by complex, cell-based assays.
Efficient screening platforms are needed to accelerate antibody discovery for therapeutic applications.

Purpose of the Study:

To develop and validate a scalable screening platform for identifying neutralizing antibodies.
To overcome limitations of traditional neutralization assays.
To demonstrate the utility of the platform in discovering antibodies against viral diseases.

Main Methods:

Development of engineered cell lines for high-throughput screening.
Implementation of Droplet Reporter Cell Testing for Neutralization (DrReCT-Neutralization).
Validation of the DrReCT-Neutralization platform using synthetic oligoclonal antibody libraries.

Main Results:

Successful establishment and validation of the DrReCT-Neutralization screening platform.
Demonstration of scalable functional antibody data collection against viral targets.
Identification of neutralizing antibodies through a discovery campaign.

Conclusions:

DrReCT-Neutralization offers a scalable and efficient method for discovering neutralizing antibodies.
The platform facilitates functional antibody data collection, accelerating the development of antiviral therapies.
This technology has broad implications for infectious disease research and therapeutic antibody discovery.