High-Throughput Antibody Neutralization Screening in Massively Parallel Droplet Arrays

Area of Science:

• Immunology
• Biotechnology
• Virology

Background:

• Neutralizing antibodies are key for rapid immune defense against infections.
• Current methods for discovering neutralizing antibodies at scale are limited by complex, cell-based assays.
• Efficient screening platforms are needed to accelerate antibody discovery for therapeutic applications.

Purpose of the Study:

• To develop and validate a scalable screening platform for identifying neutralizing antibodies.
• To overcome limitations of traditional neutralization assays.
• To demonstrate the utility of the platform in discovering antibodies against viral diseases.

Main Methods:

• Development of engineered cell lines for high-throughput screening.
• Implementation of Droplet Reporter Cell Testing for Neutralization (DrReCT-Neutralization).
• Validation of the DrReCT-Neutralization platform using synthetic oligoclonal antibody libraries.

Main Results:

• Successful establishment and validation of the DrReCT-Neutralization screening platform.
• Demonstration of scalable functional antibody data collection against viral targets.
• Identification of neutralizing antibodies through a discovery campaign.

Conclusions:

• DrReCT-Neutralization offers a scalable and efficient method for discovering neutralizing antibodies.
• The platform facilitates functional antibody data collection, accelerating the development of antiviral therapies.
• This technology has broad implications for infectious disease research and therapeutic antibody discovery.