Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Intracellular Movement of Viruses and Bacteria01:10

Intracellular Movement of Viruses and Bacteria

2.8K
Intracellular bacteria and viruses often comprise a group of highly infectious pathogens that can cause several diseases. Bacterial pathogens include those belonging to the genus Rickettsia responsible for conditions such as rocky mountain spotted fever and the Mediterranean spotted fever; Chlamydia, a genus responsible for a sexually transmitted disease; Coxiella burnetii, an agent responsible for Q fever. Viral pathogens include vaccinia—a poxvirus, and herpes simplex virus—a...
2.8K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Mutant SOD1 expressed by oligodendrocytes aggregates in myelinic nanochannels and accelerates disease progression in familial ALS mice.

bioRxiv : the preprint server for biology·2026
Same author

Intrathecal (G<sub>4</sub>C<sub>2</sub>)<sub>149</sub> delivery in C9orf72-deficient mice yields mild motor dysfunction and ALS/FTD pathological hallmarks.

Acta neuropathologica communications·2026
Same author

Enterovirus D68 2A protease causes nuclear pore complex dysfunction and independently contributes to motor neuron toxicity.

eLife·2026
Same author

Electrodiagnostic Studies as a Diagnostic and Prognostic Tool in Acute Flaccid Myelitis.

Muscle & nerve·2026
Same author

Is SORL1 a common genetic target across neurodegenerative diseases? A multi-ancestry biobank study.

Brain : a journal of neurology·2026
Same author

Functional Activity of TDP 43: A Direct Biomarker for ALS.

medRxiv : the preprint server for health sciences·2026

Related Experiment Video

Updated: May 27, 2025

Assay to Measure Nucleocytoplasmic Transport in Real Time within Motor Neuron-like NSC-34 Cells
08:53

Assay to Measure Nucleocytoplasmic Transport in Real Time within Motor Neuron-like NSC-34 Cells

Published on: May 16, 2017

8.6K

Enterovirus D68 2A protease causes nuclear pore complex dysfunction and motor neuron toxicity.

Katrina M Zinn, Mathew W McLaren, Michael T Imai

    Biorxiv : the Preprint Server for Biology
    |February 20, 2025
    PubMed
    Summary

    Enterovirus D68 proteases disrupt the nuclear pore complex (NPC), leading to motor neuron death in acute flaccid myelitis (AFM). Targeting these proteases may offer a new therapeutic strategy for AFM.

    More Related Videos

    Why Quantification Matters: Characterization of Phenotypes at the Drosophila Larval Neuromuscular Junction
    10:41

    Why Quantification Matters: Characterization of Phenotypes at the Drosophila Larval Neuromuscular Junction

    Published on: May 12, 2016

    8.1K
    Measuring RAN Peptide Toxicity in C. elegans
    10:49

    Measuring RAN Peptide Toxicity in C. elegans

    Published on: April 30, 2020

    6.6K

    Related Experiment Videos

    Last Updated: May 27, 2025

    Assay to Measure Nucleocytoplasmic Transport in Real Time within Motor Neuron-like NSC-34 Cells
    08:53

    Assay to Measure Nucleocytoplasmic Transport in Real Time within Motor Neuron-like NSC-34 Cells

    Published on: May 16, 2017

    8.6K
    Why Quantification Matters: Characterization of Phenotypes at the Drosophila Larval Neuromuscular Junction
    10:41

    Why Quantification Matters: Characterization of Phenotypes at the Drosophila Larval Neuromuscular Junction

    Published on: May 12, 2016

    8.1K
    Measuring RAN Peptide Toxicity in C. elegans
    10:49

    Measuring RAN Peptide Toxicity in C. elegans

    Published on: April 30, 2020

    6.6K

    Area of Science:

    • Virology
    • Neuroscience
    • Cell Biology

    Background:

    • Enterovirus D68 (EV-D68) causes acute flaccid myelitis (AFM), characterized by motor neuron death.
    • Mechanisms linking EV-D68 to neurotoxicity, particularly motor neuron injury, remain unclear.
    • Nuclear pore complex (NPC) dysfunction is implicated in neurodegenerative diseases and altered during picornavirus infections.

    Purpose of the Study:

    • To investigate the impact of EV-D68 proteases on NPC structure and function.
    • To determine the role of EV-D68 proteases in motor neuron toxicity.

    Main Methods:

    • Analysis of NPC composition following EV-D68 protease expression.
    • Utilized reporter systems to assess NPC function (nuclear import/export, permeability).
    • Assessed toxicity in induced pluripotent stem cell-derived motor neurons and tested a protease inhibitor.

    Main Results:

    • EV-D68 2A and 3C proteases cleave key nucleoporins, including Nup98 and POM121 by 2Apro.
    • 2Apro inhibits nuclear import/export and disrupts NPC permeability, without affecting RNA export.
    • 2Apro is toxic to motor neurons, with toxicity rescued by telaprevir at sub-antiviral concentrations.

    Conclusions:

    • EV-D68 proteases, particularly 2Apro, significantly disrupt NPC integrity and function.
    • NPC dysfunction mediated by EV-D68 proteases contributes to motor neuron toxicity in AFM.
    • Targeting EV-D68 proteases or the NPC presents a potential therapeutic avenue for AFM.