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Updated: May 27, 2025

DNA Sequence Recognition by DNA Primase Using High-Throughput Primase Profiling
Published on: October 8, 2019
URAdime - a tool for analyzing primer sequences in sequencing data to identify dimers and super-amplicons
Jason D Limberis1, John Z Metcalfe1
1Division of Pulmonary and Critical Care Medicine, Zuckerberg San Francisco General Hospital and Trauma Centre, University of California, San Francisco, San Francisco, CA, USA.
Abstract:
Targeted sequencing of multiplex PCR amplicons is commonplace in research laboratories and clinical diagnostics. There are numerous tools for the a priori optimization of primers and reactions, but no tools to detect specific problematic primers post hoc. We developed URAdime, a tool for analyzing primer sequences in sequencing data to identify dimers and super-amplicons. We show that it successfully detects these unwanted amplicons and accurately aSributes their generation to specific primers.
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