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Updated: Jun 12, 2025

The Soft Agar Colony Formation Assay
Published on: October 27, 2014
UBE2C regulating the lung carcinoma progression via inhibiting ubiquitin-proteasomal degradation to increase MMP9
Jie Zhao1, Juanjuan Dai2, Ning Zhou3
1Department of Oncology, Binzhou Medical University Hospital, Binzhou, People's Republic of China.
Background:
Non-small-cell-lung-cancer (NSCLC) is a prevalent lung malignancy among humans. UBE2C is a critical component of the ubiquitin-proteasome system, and its expression level is significantly associated with cancer development and cell proliferation. Nevertheless, the mechanisms of UBE2C in NSCLC remain unclear.
Objective:
We aimed to investigate the role of MMP9 in UBE2C-overexpressed NSCLC progression.
Methods:
The GEPIA database and Kaplan-Meier curves were used to determine UBE2C expression in human tumors and survival in NSCLC. CCK8, colony formation, and Transwell® assays were employed to assess the function of UBE2C in vitro. Western blotting, immunofluorescence assay, and RT-qPCR were utilized to determine protein and mRNA expression levels. CHX chase and co-immunoprecipitation assays were used to elucidate the regulatory mechanism.
Results:
This research proved that UBE2C expression was related to patient overall survival, cell proliferation and migration. Furthermore, overexpressed UBE2C could promote the protein stability of Matrix metalloproteinase-9 (MMP9) to upregulate its protein level in NSCLC cells. Meanwhile, UBE2C upregulation promoted lung carcinoma progression by modulating MMP9 expression.
Conclusions:
Our findings indicate that UBE2C may be a therapeutic and prognostic target for lung carcinoma.
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