Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Colloidal precipitates01:09

Colloidal precipitates

484
The high insolubility of some precipitates can result in an unfavorable relative supersaturation. This can lead to colloidal particles with a large surface-to-mass ratio, where adsorption is promoted. For instance, in the precipitation of silver chloride, silver ions are adsorbed on the surface of the colloidal particles, forming a primary layer. This layer attracts ions of opposite charge (such as nitrate ions), forming a diffuse secondary layer of adsorbed ions. This electric double layer...
484
Distillation: Vapor–Liquid Equilibria01:01

Distillation: Vapor–Liquid Equilibria

2.6K
Distillation is a separation technique that takes advantage of the boiling point properties of disparate elements in a mixture. To perform distillation, we begin by heating a miscible mixture of two liquids with a significant difference in boiling points (at least 20°C). As the solution heats up and reaches the bubble point of the more volatile component, some molecules of the more volatile component transition into the gas phase and travel upward into the condenser, which is a glass tube...
2.6K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Somatic mutations impose an entropic upper bound on human lifespan.

npj aging·2026
Same author

Treacle-dependent TOPBP1 condensation regulates the nucleolar DNA damage response.

Nucleic acids research·2026
Same author

Gut Hi-C metagenomes of severe COVID-19 patients: bacteria and yeast involved in gut-lung axis.

mSphere·2026
Same author

Extrusion fountains are hallmarks of chromosome organization emerging upon zygotic genome activation.

Nature communications·2026
Same author

Many faces of mammalian NSD methyltransferases.

Cellular & molecular biology letters·2026
Same author

Do we actually need aging clocks?

npj aging·2025

Related Experiment Video

Updated: May 27, 2025

Microfluidic Preparation of Liquid Crystalline Elastomer Actuators
12:04

Microfluidic Preparation of Liquid Crystalline Elastomer Actuators

Published on: May 20, 2018

8.9K

Liquid condensates: a new barrier to loop extrusion?

Arseniy V Selivanovskiy1,2, Maria N Molodova1,3, Ekaterina E Khrameeva3

  • 1Institute of Gene Biology, Russian Academy of Sciences, 119334, Moscow, Russia.

Cellular and Molecular Life Sciences : CMLS
|February 20, 2025
PubMed
Summary

Macromolecular condensates, formed by liquid-liquid phase separation (LLPS), may block loop extrusion (LE), a process crucial for enhancer-promoter interactions and gene regulation.

Keywords:
CohesinEnhancer-promoter communicationGenome spatial organizationLiquid–liquid phase separationLoop extrusionMacromolecule condensates

More Related Videos

Fabricating High-viscosity Droplets using Microfluidic Capillary Device with Phase-inversion Co-flow Structure
08:02

Fabricating High-viscosity Droplets using Microfluidic Capillary Device with Phase-inversion Co-flow Structure

Published on: April 17, 2018

10.3K
Author Spotlight: Developing Synthetic Cells from Programmable Amphiphilic DNA Nanostructures
08:02

Author Spotlight: Developing Synthetic Cells from Programmable Amphiphilic DNA Nanostructures

Published on: May 31, 2024

680

Related Experiment Videos

Last Updated: May 27, 2025

Microfluidic Preparation of Liquid Crystalline Elastomer Actuators
12:04

Microfluidic Preparation of Liquid Crystalline Elastomer Actuators

Published on: May 20, 2018

8.9K
Fabricating High-viscosity Droplets using Microfluidic Capillary Device with Phase-inversion Co-flow Structure
08:02

Fabricating High-viscosity Droplets using Microfluidic Capillary Device with Phase-inversion Co-flow Structure

Published on: April 17, 2018

10.3K
Author Spotlight: Developing Synthetic Cells from Programmable Amphiphilic DNA Nanostructures
08:02

Author Spotlight: Developing Synthetic Cells from Programmable Amphiphilic DNA Nanostructures

Published on: May 31, 2024

680

Area of Science:

  • Molecular Biology
  • Genetics
  • Biochemistry

Background:

  • Liquid-liquid phase separation (LLPS) drives the formation of macromolecular condensates on chromatin.
  • These condensates concentrate effector factors for transcriptional regulation and DNA repair.
  • Enhancers, super-enhancers, and promoters are key platforms for condensate assembly.

Purpose of the Study:

  • To investigate the role of condensates in regulating enhancer-promoter (EP) interactions.
  • To propose a novel mechanism where condensates act as barriers to loop extrusion (LE).

Main Methods:

  • The study integrates existing evidence on LLPS, chromatin organization, and gene regulation.
  • It proposes a theoretical model based on current understanding of condensate function and LE.

Main Results:

  • Enhancer-promoter interactions may arise from condensate contact and fusion.
  • Condensates are proposed to function as barriers to loop extrusion (LE) mediated by SMC complexes.
  • This barrier function could influence the spatial arrangement of regulatory genomic elements.

Conclusions:

  • Condensates formed via LLPS may regulate nuclear processes by modulating LE.
  • This mechanism provides a new perspective on how chromatin architecture and gene regulation are linked.
  • Further experimental validation is needed to confirm the role of condensates as LE barriers.