Related Experiment Video

Updated: May 11, 2026

Investigating the Effects of Probiotics on Pneumococcal Colonization Using an In Vitro Adherence Assay
09:20

Investigating the Effects of Probiotics on Pneumococcal Colonization Using an In Vitro Adherence Assay

Published on: April 28, 2014

12.5K

Genomic and In Vitro Analysis of Pediococcus pentosaceus MBBL4 Implicated Its Therapeutic Use Against Mastitis

Md Morshedur Rahman1, Naim Siddique1, Md Abu Ahsan Gilman1

  • 1Molecular Biology and Bioinformatics Laboratory, Department of Gynecology, Obstetrics and Reproductive Health, Bangabandhu Sheikh Mujibur Rahman Agricultural University, Gazipur, 1706, Bangladesh.

Probiotics and Antimicrobial Proteins
|February 20, 2025
PubMed

Insights

Pediococcus pentosaceus MBBL4, a cow milk isolate, shows strong probiotic potential with extensive carbohydrate metabolism and antimicrobial compound production. Its genome analysis reveals safety and efficacy against mastitis pathogens, positioning it as a novel biologic candidate.

Area of Science:

  • Microbiology and Genomics
  • Probiotics and Bioactive Compounds

Background:

  • Rising antimicrobial resistance (AMR) and non-communicable diseases necessitate novel probiotics and biologics.
  • Pediococcus pentosaceus strains are explored for their therapeutic potential.
  • Understanding the genomic basis of probiotic traits is crucial for strain selection.

Purpose of the Study:

  • To analyze the whole genome of Pediococcus pentosaceus MBBL4 for probiotic properties and antimicrobial efficacy.
  • To assess its carbohydrate utilization, metabolic pathways, genomic safety, and potential as a therapeutic agent.
  • To evaluate its in vitro antimicrobial activity against bovine mastitis pathogens.

Main Methods:

  • Whole genome sequencing (WGS), assembly, and annotation of P. pentosaceus MBBL4.
  • Phylogenetic and comparative genomic analyses.
  • In vitro antimicrobial efficacy testing against Staphylococcus aureus and Escherichia coli.

Main Results:

  • WGS revealed significant probiotic traits, including extensive carbohydrate metabolism (e.g., lactose pathway) and gallic acid metabolism.
  • MBBL4 genome encodes antimicrobial compounds (enterocin A, lactococcin, penocin A) and novel exopolysaccharide biosynthesis proteins.
  • The strain demonstrated sensitivity to most tested antibiotics, possessing only a single resistance gene (lnuA), and inhibited key mastitis pathogens.
  • Low pathogenicity score supports a favorable safety profile.

Conclusions:

  • Pediococcus pentosaceus MBBL4 possesses a robust genomic profile supporting its use as a probiotic and biologic.
  • The strain exhibits promising antimicrobial efficacy against mastitis pathogens, indicating potential as a natural therapeutic.
  • MBBL4 represents a valuable candidate for developing novel interventions against AMR and mastitis.
Keywords:
Pediococcus pentosaceusBacteriocinsCow milkLactose metabolismProbiotics

More Related Videos

Using the Overlay Assay to Qualitatively Measure Bacterial Production of and Sensitivity to Pneumococcal Bacteriocins06:05

Using the Overlay Assay to Qualitatively Measure Bacterial Production of and Sensitivity to Pneumococcal Bacteriocins

Published on: September 30, 2014

13.5K
Analysis of Interactions between Endobiotics and Human Gut Microbiota Using In Vitro Bath Fermentation Systems00:06

Analysis of Interactions between Endobiotics and Human Gut Microbiota Using In Vitro Bath Fermentation Systems

Published on: August 23, 2019

6.8K

Related Concept Videos

Production of Antibiotics01:27

Production of Antibiotics

Penicillin, one of the earliest and most widely used antibiotics, is produced industrially by the filamentous fungus Penicillium chrysogenum. Large stirred-tank bioreactors ranging from tens to hundreds of thousands of liters maintain tightly controlled temperature, pH, and dissolved oxygen conditions to support fungal metabolism and maximize antibiotic yield. Penicillin is a secondary metabolite, synthesized primarily during the stationary growth phase, which requires a carefully managed...
Mechanism of Antibiotic Resistance in MRSA01:25

Mechanism of Antibiotic Resistance in MRSA

Antibiotic resistance in bacteria arises when microorganisms evolve the ability to withstand drugs designed to kill them or inhibit their growth, rendering once-effective treatments useless. This phenomenon, driven by genetic change and selection under antibiotic exposure, poses a profound threat to modern medicine. Mechanisms include drug-inactivating enzymes (e.g., β-lactamases), efflux pumps that eject antibiotics, mutations altering antibiotic targets, decreased drug uptake, and acquisition...
Clinical Significance of Antibiotic Resistance01:25

Clinical Significance of Antibiotic Resistance

Methicillin-resistant Staphylococcus aureus (MRSA) presents a critical public health threat, arising from its capacity to resist β-lactam antibiotics due to acquisition of the mecA gene within the staphylococcal cassette chromosome mec (SCCmec). This gene encodes penicillin-binding protein 2a (PBP2a), which impairs binding efficacy of methicillin and other β-lactams. MRSA has evolved into distinct clonal lineages impacting humans and animals alike, reinforcing its significance within the One...