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Enhancing lipase enzymatic performance with dynamic covalent dextran-based hydrogels.

Meng Sun1, Jie Gao1, Yingying Zhao1

  • 1College of Life Science, Xinyang Normal University, Xinyang 464000, China.

International Journal of Biological Macromolecules
|February 20, 2025
PubMed
Summary

This study presents a novel polysaccharide-based dynamic hydrogel for immobilizing lipase (an enzyme). This enzyme immobilization enhances stability and catalytic activity, crucial for industrial biocatalysis applications.

Keywords:
CarboxymethylchitosanCatalytic activityLipaseOxidized dextransStability

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Area of Science:

  • Biochemistry
  • Materials Science
  • Chemical Engineering

Background:

  • Maintaining high catalytic activity and stability is essential for efficient biocatalysis.
  • Enzyme immobilization in hydrogel systems is a key strategy to achieve this.
  • Natural polymer interactions inspire advanced hydrogel designs.

Purpose of the Study:

  • To develop a novel polysaccharide-based dynamic hydrogel for enzyme immobilization.
  • To enhance the stability and catalytic performance of lipase.
  • To explore applications in industrial biocatalysis.

Main Methods:

  • Synthesized a dynamic hydrogel using oxidized dextrans (ODex) and carboxymethylchitosan.
  • Immobilized lipase within the hydrogel via Schiff base reaction (imine bonds).
  • Characterized the hydrogel and immobilized lipase using FT-IR, 1H NMR, XRD, and hydroxylamine hydrochloride method.

Main Results:

  • Successfully prepared ODex and immobilized lipase.
  • The hydrogel significantly improved lipase storage stability, thermal stability, and reusability.
  • Immobilized lipase demonstrated enhanced activity and stability under harsh conditions (high temperature, strong base, organic solvents).
  • Achieved a higher degree of triacylglycerol hydrolysis.

Conclusions:

  • The developed polysaccharide-based dynamic hydrogel is a promising platform for enzyme immobilization.
  • This system offers improved enzyme performance and stability for industrial biocatalysis.
  • The hydrogel system has versatile applications in various biocatalytic processes.