Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Endothelial cell density determined by specular microscopy and scanning electron microscopy.

P S Binder, P Akers, E Y Zavala

    Ophthalmology
    |October 1, 1979
    PubMed
    Summary

    Specular microscopy accurately measures normal corneal endothelial cell density. However, for non-uniform cell populations, scanning electron microscopy with larger, varied sample areas improves accuracy in corneal cell density analysis.

    Related Concept Videos

    You might also read

    Related Articles

    Articles linked to this work by shared authors, journal, and citation graph.

    Sort by
    Same author

    Early institutional experience with third-party procurement in lung transplantation-an observational pilot study.

    The Journal of thoracic and cardiovascular surgery·2025
    Same author

    Consensus conference on best practices in live kidney donation: recommendations to optimize education, access, and care.

    American journal of transplantation : official journal of the American Society of Transplantation and the American Society of Transplant Surgeons·2015
    Same author

    Concomitant chemotherapy and radiation for the treatment of advanced-stage endometrial cancer.

    Gynecologic oncology·2014
    Same author

    Dynamic challenges inhibiting optimal adoption of kidney paired donation: findings of a consensus conference.

    American journal of transplantation : official journal of the American Society of Transplantation and the American Society of Transplant Surgeons·2013
    Same author

    A web-based application for initial screening of living kidney donors: development, implementation and evaluation.

    American journal of transplantation : official journal of the American Society of Transplantation and the American Society of Transplant Surgeons·2012
    Same author

    New method for reshaping the cornea.

    Journal of biomedical optics·2012

    Area of Science:

    • Ophthalmology
    • Microscopy
    • Cell Biology

    Background:

    • Corneal endothelial cell density is crucial for ocular health.
    • Accurate cell counting is essential for diagnosing and managing corneal diseases.
    • Existing microscopy techniques have limitations in assessing cell density in diverse corneal conditions.

    Purpose of the Study:

    • To compare specular microscopy and scanning electron microscopy for corneal endothelial cell analysis.
    • To determine the accuracy of specular microscopy in normal versus non-homogeneous corneal populations.
    • To identify methods for improving the accuracy of corneal cell density measurements.

    Main Methods:

    • Human corneas were analyzed using specular microscopy and scanning electron microscopy.
    • Corneal shrinkage during sample processing for scanning electron microscopy was quantified.

    Related Experiment Videos

  • Cell counts were performed using a planimeter on all specimens.
  • Different sampling strategies were tested to optimize accuracy.

    • Main Results:

    • Specular microscopy accurately assessed endothelial cell density in normal central and paracentral corneas.
    • Specular microscopy showed significant error in non-homogeneous populations (e.g., post-surgery).
    • Peripheral corneal endothelial cell density was lower than central density.
    • Using a variable frame, multiple photographs from different areas, and larger sample sizes improved counting accuracy.

    Conclusions:

    • Specular microscopy is reliable for normal corneas but less so for non-homogeneous populations.
    • Scanning electron microscopy, with optimized sampling, offers improved accuracy for complex corneal conditions.
    • Adjusting microscopic techniques based on corneal homogeneity is vital for precise endothelial cell density assessment.