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Antimicrobial Synergy Testing by the Inkjet Printer-assisted Automated Checkerboard Array and the Manual Time-kill Method
Published on: April 18, 2019
Synergistic antibacterial activity of chitosan-polyethylene glycol nanocomposites films containing ZIF-8 and
Fahimeh Jamiri1, Bahar Nayeri Fasaei2, Seyed Mehdi Joghataei1
1Department of Microbiology and Immunology, Faculty of Veterinary Medicine, University of Tehran, Tehran, Iran.
Background:
Antibiotic resistance is a growing global threat due to antibiotic overuse and limited treatment options. Multidrug-resistant bacteria, like Staphylococcus aureus and Escherichia coli, increase infection complexity and mortality. This study explores nanocomposite films of ZIF-8 nanoparticles and Doxycycline (Dox) to enhance antibacterial efficacy. In this study, nanocomposite films composed of chitosan (CS) and polyethylene glycol (PEG), incorporating zeolitic imidazolate framework-8 (ZIF-8) nanoparticles and DOX, were developed. These films were characterized by their morphological, mechanical, antibacterial, and drug-release properties. Antibacterial efficacy was evaluated using disk diffusion, broth microdilution, and checkerboard assay methods to determine MICs and potential synergistic effects.
Results:
The nanocomposite films demonstrated flexibility, semi-transparency, and a yellowish-brown hue, with films containing ZIF-8 nanoparticles being thicker (79 ± 0.2 μm) than those without (54 ± 0.5 μm). The tensile strength was enhanced with the incorporation of ZIF-8, peaking at 53.12 MPa for the CS-PEG-G-10% DOX-4% ZIF-8 film. XRD analysis confirmed the crystallinity of the ZIF-8 and DOX, with distinct peaks observed for each material. The drug release studies revealed an initial burst followed by sustained release, with higher release rates in acidic environments compared to neutral and alkaline media. The CS-PEG-G-10% DOX-4% ZIF-8 nanocomposite film demonstrated significantly higher antibacterial activity, achieving the lowest MIC values, particularly against S. aureus (22.5 mm inhibition zone) compared to E. coli (14 mm inhibition zone). Additionally, a notable synergistic effect was observed between CS-PEG-G-10% DOX and CS-PEG-G-10% DOX, with FICI values below 0.5.
Conclusions:
The CS-PEG-G-10% DOX-4% ZIF-8 nanocomposite exhibits enhanced antibacterial efficacy and optimal properties, positioning it as a strong candidate for developing effective treatments against multidrug-resistant pathogens.
Insights
This study developed novel nanocomposite films combining chitosan, polyethylene glycol, ZIF-8 nanoparticles, and Doxycycline to combat antibiotic resistance. The enhanced films show superior antibacterial activity against multidrug-resistant bacteria like Staphylococcus aureus.
Area of Science:
- Materials Science
- Nanotechnology
- Biomedical Engineering
Background:
- Antibiotic resistance poses a significant global health threat, necessitating novel therapeutic strategies.
- Multidrug-resistant bacteria, including Staphylococcus aureus and Escherichia coli, complicate infections and increase mortality rates.
- This research addresses the need for advanced antibacterial agents by exploring nanocomposite materials.
Purpose of the Study:
- To develop and characterize novel nanocomposite films incorporating zeolitic imidazolate framework-8 (ZIF-8) nanoparticles and Doxycycline (Dox).
- To evaluate the morphological, mechanical, drug-release, and antibacterial properties of these nanocomposite films.
- To assess the potential of these materials as effective treatments against multidrug-resistant pathogens.
Main Methods:
- Nanocomposite films were fabricated using chitosan (CS) and polyethylene glycol (PEG) with ZIF-8 nanoparticles and Doxycycline.
- Characterization included morphological, mechanical (tensile strength), and structural (XRD) analyses.
- Antibacterial efficacy was determined using disk diffusion, broth microdilution (MIC), and checkerboard assays to identify synergistic effects.
Main Results:
- The CS-PEG-G-10% DOX-4% ZIF-8 nanocomposite films exhibited enhanced tensile strength and controlled drug release profiles.
- XRD confirmed the crystallinity of ZIF-8 and Doxycycline within the nanocomposite structure.
- The nanocomposite films demonstrated significant antibacterial activity, particularly against Staphylococcus aureus, with observed synergistic effects.
Conclusions:
- The developed CS-PEG-G-10% DOX-4% ZIF-8 nanocomposite films possess enhanced antibacterial efficacy and favorable material properties.
- These nanocomposites represent a promising platform for developing advanced treatments against multidrug-resistant bacteria.
- Further development could lead to effective therapeutic solutions for challenging infections.
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