Isolation and quantitation of carbohydrates in sheep plasma by high-performance liquid chromatography

The application of ultraviolet detection at 190 nm following chromatography on a Ca2+ cation-exchange column with a mobile phase of water enables the low amounts of carbohydrates present in plasma to be quantitated. The separation and quantitation of carbohydrates in maternal and fetal sheep plasma and amniotic fluid are described, as is the application of this method to the determination of specific radioactivities of glucose and fructose in plasma.