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Advancing super-resolution microscopy with metal complexes: functional imaging agents for nanoscale visualization.

Nur Aininie Yusoh¹, Martin R Gill², Xiaohe Tian¹

¹Department of Radiology, Huaxi MR Research Center (HMRRC), Institution of Radiology and Medical Imaging, West China Hospital of Sichuan University, Sichuan University, Chengdu, Sichuan, China. xiaohe.t@wchscu.cn.

Chemical Society Reviews

|February 21, 2025

Summary

This summary is machine-generated.

Metal complexes offer superior photostability and tuneable luminescence for advanced super-resolution microscopy (SRM). These probes enable deep-tissue imaging and theranostic applications, overcoming limitations of organic fluorescent probes.

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Area of Science:

Bioimaging
Nanotechnology
Materials Science

Background:

Super-resolution microscopy (SRM) provides nanoscale visualization but is limited by organic probes' photobleaching and instability.
Existing probes perform poorly in deep-tissue imaging, restricting biological process observation.
Metal complexes offer enhanced photostability, tuneable luminescence, and long excited-state lifetimes.

Purpose of the Study:

To review recent advancements in applying metal complexes for super-resolution microscopy.
To highlight the utility of metal complexes in visualizing subcellular structures and live-cell dynamics.
To discuss the theranostic potential of metal complexes in disease diagnostics and treatment.

Main Methods:

Exploration of rational design strategies for metal complexes to enhance biocompatibility and specificity.
Discussion of metal nanoparticles (NPs) and hybrid nanoscale probes for SRM applications.
Analysis of metal complex performance in deep-tissue penetration and multiplexed imaging.

Main Results:

Metal complexes overcome photobleaching and photostability issues associated with organic probes.
These complexes enable precise subcellular targeting, long-term imaging, and visualization of intricate structures.
Metal complexes facilitate real-time diagnostics and treatments, showing theranostic potential for diseases.

Conclusions:

Metal complexes are versatile tools for advancing super-resolution microscopy.
Rational design optimizes metal complexes for improved biocompatibility, specificity, and deep-tissue imaging.
Emerging hybrid probes offer promising solutions for overcoming current SRM limitations.