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Xiao-Chai-Hu-Tang Ameliorates Depressive Symptoms via Modulating Neuro-Endocrine Network in Chronic Unpredictable
Ying Feng1,2, Wenkai Wang1, Yingru Zhang1,2,3
1Department of Medical Oncology, Shuguang Hospital, Shanghai University of Traditional Chinese Medicine, Shanghai, China.
CNS Neuroscience & Therapeutics
|February 21, 2025
Summary
Xiao-Chai-Hu-Tang (XCHT) demonstrates antidepressant effects by reducing neuroinflammation and promoting nerve regeneration. This traditional Chinese medicine modulates key signaling pathways involved in depression.
Area of Science:
- Traditional Chinese Medicine
- Neuroscience
- Pharmacology
Background:
- Xiao-Chai-Hu-Tang (XCHT) is clinically used for depression, but its mechanisms are unclear.
- Chronic unpredictable mild stress (CUMS) induces depressive-like behaviors in mice, serving as a model for investigation.
Purpose of the Study:
- To investigate XCHT's effects on CUMS-induced depressive behaviors in mice.
- To elucidate the underlying molecular mechanisms of XCHT's antidepressant action.
Main Methods:
- Integrated analysis of UPLC-Q-TOF-MS, network pharmacology, and bioinformatics to identify XCHT active components and targets.
- Utilized PPI, GO, KEGG, and molecular docking for mechanism prediction.
- Experimental validation included ELISA, metabolomics, RNA-seq, RT-PCR, immunofluorescence, and Western blotting.
Main Results:
- Identified 166 active ingredients and 525 targets for XCHT.
- XCHT exhibited antidepressant effects, reduced quinoline acid levels, and modulated neuroinflammation.
- XCHT promoted nerve regeneration via BDNF/TrkB/CREB and PI3K/AKT pathways, downregulating microglial and astrocyte activation.
Conclusions:
- XCHT exerts antidepressant effects by modulating neuroinflammation.
- XCHT promotes neuroregeneration, contributing to its antidepressant functions.
