Insecticide resistance management scenarios differ for RNA-based sprays and traits

Area of Science:

• Agricultural Entomology
• Molecular Biology
• Pest Management

Background:

• RNA-based bioinsecticides utilizing double-stranded RNA (dsRNA) and RNA interference (RNAi) are emerging as sustainable pest control solutions.
• These RNAi technologies present a safer and more specific alternative to traditional chemical insecticides, benefiting both human and environmental health.

Purpose of the Study:

• To outline essential insect resistance management (IRM) programs for RNA interference (RNAi) based bioinsecticides.
• To differentiate IRM strategies for dsRNA insecticidal traits in transgenic crops versus sprayable dsRNA biopesticides.

Main Methods:

• IRM programs for RNAi products align with existing guidelines for insecticidal traits and conventional sprays.
• Strategies for dsRNA traits involve pyramiding non-cross-resistant traits and incorporating refuges of non-transgenic plants.
• IRM for dsRNA sprays focuses on application timing to target a single pest generation and rotating with different IRAC mode of action groups.

Main Results:

• Continuous exposure of dsRNA traits in crops requires pyramiding and refuges for resistance management.
• Intermittent exposure of dsRNA sprays necessitates strategic application timing and rotation with other insecticide classes.
• Both approaches aim to mitigate resistance development and prolong the efficacy of RNAi technologies.

Conclusions:

• Tailored IRM strategies are vital for the sustainable deployment of RNAi insecticidal technologies.
• Effective resistance management ensures the longevity and environmental benefits of dsRNA-based bioinsecticides in agriculture.
• Implementing distinct IRM plans for transgenic traits and sprayable formulations is key to managing pest resistance.